|
| Status |
Public on May 31, 2018 |
| Title |
T24 cells_NF90 SiRNA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
human bladder cancer cells T24, transfected with NF90 siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: T24
transfection: NF90 siRNA
|
| Treatment protocol |
T24 cells were transfected with 20μM siRNA control (siNC) or siRNA NF90 (siNF90) for 48 hours using Lipofectamine 3000. Then, cells were washed with PBS and placed on ice in the Trizol solution.
|
| Growth protocol |
Cells were maintained in RPMI1640 supplemented with 10% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 “C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays).
|
| |
|
| Hybridization protocol |
Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
|
| Description |
human bladder cancer cells T24, transfected with NF90 siRNA
|
| Data processing |
The data were analyzed with Robust Multi-chip Average (RMA) algorithm as normalization method.
|
| |
|
| Submission date |
May 30, 2016 |
| Last update date |
May 31, 2018 |
| Contact name |
Junlong Zhuang |
| E-mail(s) |
zhuangjl-2008@163.com
|
| Organization name |
Institute of Urology, Drum Tower hospital
|
| Street address |
321# Zhongshan Road
|
| City |
Nanjing |
| ZIP/Postal code |
210008 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE82024 |
Expression data from human T24 cells transfected with siNC or siNF90 |
|
