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Sample GSM218357 Query DataSets for GSM218357
Status Public on Jul 08, 2008
Title Lethal Yellow mice control #1
Sample type RNA
 
Source name ovary
Organism Mus musculus
Characteristics Ovary from control obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with vehicle (0.1 ml 100% DMSO) from 120 to 180 days of age.
Extracted molecule total RNA
Extraction protocol The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
Label Alexa-647
Label protocol see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
 
Hybridization protocol CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
Scan protocol Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
Description IACUC approval was obtained for all animal treatments.
Data processing global median normalization, CodeLink Expression Analysis 4.1.0.4163
 
Submission date Aug 15, 2007
Last update date Feb 12, 2009
Contact name Kathleen M Eyster
E-mail(s) Kathleen.Eyster@usd.edu
Organization name University of South Dakota
Department Basic Biomedical Sciences
Street address 414 E. Clark St.
City Vermillion
State/province SD
ZIP/Postal code 57069
Country USA
 
Platform ID GPL2897
Series (1)
GSE8806 Pioglitazone treatment of Lethal Yellow mice

Data table header descriptions
ID_REF
Raw_intensity Raw signal intensity value
VALUE Median normalized signal intensity value
Quality_flag CodeLink quality flag
Signal_strength Signal strength
Spot_mean Spot mean
Spot_median Spot median
Spot_stdev Spot standard deviation
Spot_area Spot area
Spot_diameter Spot diameter
Spot_noise_level Spot noise level
Bkgd_mean Background mean
Bkgd_median Background median
Bkgd_stdev Background standard deviation
Bkgd_area Background area

Data table
ID_REF Raw_intensity VALUE Quality_flag Signal_strength Spot_mean Spot_median Spot_stdev Spot_area Spot_diameter Spot_noise_level Bkgd_mean Bkgd_median Bkgd_stdev Bkgd_area
1001 6410.9780 51.2938 G 89.1219 6451.9780 6709.0000 5482.5762 140 13.3511619567871 72.3950074053744 46.5042 41.0000 20.9300 123
1002 2237.1260 17.8991 G 30.9567 2283.1260 2700.0000 1310.5740 137 13.2073392868042 73.7522508623418 48.8830 46.0000 18.5015 194
1003 42.4388 0.3395 G 1.2023 89.4388 83.0000 37.5383 100 11.2837915420532 74.3883034958893 49.1582 47.0000 18.2589 164
1004 115.1300 0.9211 G 2.3105 155.1300 147.0000 64.9154 102 11.396071434021 67.1413023771376 46.0000 40.0000 18.0942 124
1005 200.1507 1.6014 G 3.2176 248.1507 236.0000 109.6475 73 9.64087581634521 77.1226404972273 50.5138 48.0000 19.4151 187
1006 26.7692 0.2142 L 0.9474 70.7692 76.0000 27.7160 39 7.04672574996948 74.6952119373359 47.7278 44.0000 20.4635 186
1007 7127.1729 57.0240 G 89.4815 7174.1729 8190.0000 5893.6567 135 13.1105813980103 80.174902159462 52.3500 47.0000 22.1166 124
1008 7233.3286 57.8733 G 103.9991 7273.3286 7324.0000 5955.5498 139 13.303394317627 69.9364317979661 45.2917 40.0000 19.9576 124
1010 298.6491 2.3895 G 4.7588 342.6491 335.0000 117.8844 116 12.1530151367188 72.0025442819488 48.0364 44.0000 18.6684 114
1012 1162.8682 9.3040 G 17.0210 1205.8682 1363.0000 568.8281 91 10.7640514373779 70.8457474882692 46.9480 43.0000 18.5638 179
1013 71.0580 0.5685 G 1.5803 117.0580 111.0000 46.6109 69 9.37302112579346 74.0732471322223 48.8743 46.0000 18.7155 181
1014 7586.1279 60.6961 G 95.3107 7633.1279 8232.0000 6025.1880 135 13.1105813980103 80.0868280139264 51.6635 47.0000 22.0579 108
1015 7357.2129 58.8645 G 101.5792 7399.2129 8554.0000 6127.7588 143 13.4934520721436 72.8418433185123 47.0500 42.0000 20.5612 124
1017 11.4506 0.0916 L 0.7634 57.4506 53.0000 28.2661 91 10.7640514373779 75.252758893546 48.7500 46.0000 19.5018 170
1018 60.8542 0.4869 G 1.4799 109.8542 102.0000 42.6309 96 11.0558128356934 74.2322072397553 48.9811 49.0000 16.8215 110
1019 109.5342 0.8764 G 1.9170 161.5342 146.0000 72.4488 73 9.64087581634521 84.2626677024912 54.5359 52.0000 21.5084 187
1020 32.8539 0.2629 L 0.9959 79.8539 72.0000 35.0141 89 10.6451082229614 80.1812177881809 52.7885 47.0000 22.1208 216
1021 6986.6284 55.8995 G 87.9797 7034.6284 8020.0000 5990.0479 142 13.4461898803711 79.9574264685988 53.4018 48.0000 21.3050 116
1022 6629.6904 53.0437 G 87.3540 6674.6904 6986.0000 5440.4028 128 12.7661533355713 76.4096679168926 49.7750 45.0000 20.9398 124
1024 30.9744 0.2478 L 0.9673 76.9744 76.0000 33.9664 39 7.04672574996948 79.57741189421 49.2867 46.0000 22.3849 147

Total number of rows: 36227

Table truncated, full table size 4346 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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