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Sample GSM218360 Query DataSets for GSM218360
Status Public on Jul 08, 2008
Title Lethal Yellow mice control #4
Sample type RNA
 
Source name ovary
Organism Mus musculus
Characteristics Ovary from control obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with vehicle (0.1 ml 100% DMSO) from 120 to 180 days of age.
Extracted molecule total RNA
Extraction protocol The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
Label Alexa-647
Label protocol see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
 
Hybridization protocol CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
Scan protocol Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
Description IACUC approval was obtained for all animal treatments.
Data processing global median normalization, CodeLink Expression Analysis 4.1.0.4163
 
Submission date Aug 15, 2007
Last update date Feb 12, 2009
Contact name Kathleen M Eyster
E-mail(s) Kathleen.Eyster@usd.edu
Organization name University of South Dakota
Department Basic Biomedical Sciences
Street address 414 E. Clark St.
City Vermillion
State/province SD
ZIP/Postal code 57069
Country USA
 
Platform ID GPL2897
Series (1)
GSE8806 Pioglitazone treatment of Lethal Yellow mice

Data table header descriptions
ID_REF
Raw_intensity Raw signal intensity value
VALUE Median normalized signal intensity value
Quality_flag CodeLink quality flag
Signal_strength Signal strength
Spot_mean Spot mean
Spot_median Spot median
Spot_stdev Spot standard deviation
Spot_area Spot area
Spot_diameter Spot diameter
Spot_noise_level Spot noise level
Bkgd_mean Background mean
Bkgd_median Background median
Bkgd_stdev Background standard deviation
Bkgd_area Background area

Data table
ID_REF Raw_intensity VALUE Quality_flag Signal_strength Spot_mean Spot_median Spot_stdev Spot_area Spot_diameter Spot_noise_level Bkgd_mean Bkgd_median Bkgd_stdev Bkgd_area
1001 7014.8047 40.3998 G 88.7065 7060.8047 7854.0000 6046.3018 135 13.1105813980103 79.5973996586375 48.3667 46.0000 22.3983 124
1002 2772.1904 15.9657 G 36.5642 2817.1904 3325.0000 1516.6881 128 12.7661533355713 77.0477126254578 49.3250 45.0000 21.3651 124
1003 70.0303 0.4033 G 1.4938 120.0303 121.0000 40.8890 66 9.16699600219727 80.3505983319038 53.5000 50.0000 20.2337 188
1004 172.9899 0.9963 G 2.8852 218.9899 208.0000 88.3107 99 11.2272310256958 75.9011902265144 50.6750 46.0000 19.9341 124
1005 253.3067 1.4588 G 3.7574 300.3067 321.0000 118.9762 75 9.77204990386963 79.9240563211979 51.8956 47.0000 21.9494 188
1006 29.0822 0.1675 L 0.9508 73.0822 70.0000 33.4909 73 9.64087581634521 76.8617267043655 49.9545 44.0000 21.9078 114
1007 7103.7925 40.9123 G 90.2998 7152.7925 7075.0000 6088.9297 132 12.9640893936157 79.2115985287658 51.7455 49.0000 20.1411 114
1008 7407.0679 42.6589 G 86.4493 7459.0679 8091.0000 6088.8232 135 13.1105813980103 86.2825684005668 56.7833 52.0000 22.8550 124
1010 334.3773 1.9258 G 4.0714 392.3773 393.0000 131.2993 108 11.7264604568481 96.3740565648261 62.2474 58.0000 25.5827 101
1012 1179.3636 6.7922 G 15.5617 1227.3636 1328.0000 532.3427 88 10.5851345062256 78.8709453121582 52.3681 48.0000 20.5806 188
1013 104.6712 0.6028 G 1.9126 155.6712 150.0000 46.7503 73 9.64087581634521 81.3937852098806 52.9111 51.0000 20.2625 186
1014 7455.7559 42.9393 G 84.7542 7508.7559 7487.0000 6133.1948 133 13.0131034851074 88.5944867288438 57.5583 53.0000 23.7297 124
1015 7295.7998 42.0181 G 96.8607 7340.7998 9050.0000 6109.0415 142 13.4461898803711 75.7871712437444 49.0000 45.0000 20.5248 124
1017 12.1964 0.0702 L 0.7231 61.1964 51.0000 32.4353 56 8.444016456604 84.6253158595298 54.3958 49.0000 23.7502 98
1018 80.0465 0.4610 G 1.6316 128.0465 127.0000 47.7135 86 10.4641580581665 78.4811344376674 50.2586 48.0000 20.3208 120
1019 100.3333 0.5778 G 1.8858 143.3333 141.0000 44.4572 63 8.95623207092285 76.0059178016242 47.4479 43.0000 22.0039 169
1020 17.6000 0.1014 L 0.8198 70.6000 62.0000 37.5658 55 8.36828422546387 86.11395350156 56.4495 53.0000 22.0760 113
1021 8493.6553 48.9168 G 97.6997 8548.6553 11937.0000 6032.5234 118 12.2573356628418 87.4992791490163 56.3846 55.0000 21.6662 188
1022 6946.2983 40.0053 G 86.0433 6994.2983 8280.0000 5773.4331 126 12.6660251617432 81.2881690951857 51.7083 48.0000 22.1921 124
1024 9.9677 0.0574 L 0.7042 61.9677 53.0000 26.8190 31 6.2825493812561 87.992182958053 54.8229 52.0000 23.9948 100

Total number of rows: 36227

Table truncated, full table size 4361 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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