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Sample GSM218361 Query DataSets for GSM218361
Status Public on Jul 08, 2008
Title Lethal Yellow mice pioglitazone treated #1
Sample type RNA
 
Source name ovary
Organism Mus musculus
Characteristics Ovary from pioglitazone-treated obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with pioglitazone (0.1 mg/day) from 120 to 180 days of age.
Extracted molecule total RNA
Extraction protocol The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
Label Alexa-647
Label protocol see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
 
Hybridization protocol CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
Scan protocol Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
Description IACUC approval was obtained for all animal treatments.
Data processing global median normalization, CodeLink Expression Analysis 4.1.0.4163
 
Submission date Aug 15, 2007
Last update date Feb 12, 2009
Contact name Kathleen M Eyster
E-mail(s) Kathleen.Eyster@usd.edu
Organization name University of South Dakota
Department Basic Biomedical Sciences
Street address 414 E. Clark St.
City Vermillion
State/province SD
ZIP/Postal code 57069
Country USA
 
Platform ID GPL2897
Series (1)
GSE8806 Pioglitazone treatment of Lethal Yellow mice

Data table header descriptions
ID_REF
Raw_intensity Raw signal intensity value
VALUE Median normalized signal intensity value
Quality_flag CodeLink quality flag
Signal_strength Signal strength
Spot_mean Spot mean
Spot_median Spot median
Spot_stdev Spot standard deviation
Spot_area Spot area
Spot_diameter Spot diameter
Spot_noise_level Spot noise level
Bkgd_mean Background mean
Bkgd_median Background median
Bkgd_stdev Background standard deviation
Bkgd_area Background area

Data table
ID_REF Raw_intensity VALUE Quality_flag Signal_strength Spot_mean Spot_median Spot_stdev Spot_area Spot_diameter Spot_noise_level Bkgd_mean Bkgd_median Bkgd_stdev Bkgd_area
1001 7476.5244 60.4536 G 88.4463 7530.5244 6332.0000 6759.1001 126 12.6660251617432 85.1422931000767 55.7750 54.0000 20.7615 124
1002 2621.6777 21.1983 G 32.1474 2668.6777 2959.0000 1388.9702 123 12.5143299102783 83.0137084937602 53.7434 47.0000 24.0091 117
1003 35.8784 0.2901 G 1.0144 86.8784 84.0000 32.9303 74 9.70668506622314 85.6413360773144 54.1379 51.0000 23.0942 120
1004 134.2500 1.0855 G 2.1621 187.2500 179.0000 63.4595 96 11.0558128356934 86.6052393857272 55.0167 53.0000 22.4035 124
1005 205.4091 1.6609 G 2.9227 255.4091 250.0000 90.1682 66 9.16699600219727 87.3871158124861 54.3297 50.0000 24.9247 188
1006 21.2821 0.1721 L 0.8681 70.2821 56.0000 33.3497 39 7.04672574996948 80.9579883778723 54.2284 49.0000 21.3053 168
1007 7836.8320 63.3670 G 83.6737 7890.8320 9000.0000 6532.4355 121 12.4121704101563 94.3048161996076 58.0270 54.0000 26.8699 115
1008 8078.4961 65.3210 G 99.7904 8125.4961 7375.0000 6913.5039 127 12.7161874771118 81.4256372037151 52.0660 47.0000 22.9504 110
1010 323.5094 2.6158 G 4.1960 380.5094 389.0000 115.7384 108 11.7264604568481 90.684547516007 57.5294 57.0000 22.4564 106
1012 1146.5530 9.2708 G 13.0737 1197.5530 1366.0000 553.0460 85 10.4031419754028 91.6002713886918 58.6429 51.0000 27.0668 188
1013 72.4717 0.5860 G 1.4042 125.4717 125.0000 48.6119 53 8.21472454071045 89.3539485911211 58.5828 53.0000 24.2360 157
1014 8199.1055 66.2962 G 91.8987 8254.1055 9835.0000 6936.6333 125 12.6156625747681 89.8174151699548 57.5299 55.0000 23.2116 121
1015 8715.4912 70.4716 G 96.1487 8773.4912 11172.0000 6929.4146 118 12.2573356628418 91.2492386368433 58.9560 58.0000 22.1662 188
1017 17.3889 0.1406 L 0.8351 68.3889 63.0000 36.9972 36 6.7702751159668 81.8930264298098 54.0000 51.0000 20.5954 184
1018 45.1343 0.3649 G 1.2186 96.1343 97.0000 40.4600 67 9.23618125915527 78.887768966963 50.9000 51.0000 18.5918 124
1019 109.6901 0.8869 G 1.9775 159.6901 151.0000 59.2083 71 9.50789165496826 80.7539577685306 53.6538 50.0000 20.5026 188
1020 46.6800 0.3774 G 1.1627 96.6800 92.0000 34.0649 50 7.97884559631348 83.1545643670442 54.1160 50.0000 22.1030 187
1021 8454.5459 68.3617 G 108.4916 8502.5459 8545.0000 6841.9814 121 12.4121704101563 78.3705732532989 51.4167 48.0000 20.2471 186
1022 7375.3076 59.6352 G 95.2140 7423.3076 7280.0000 6353.9175 119 12.3091630935669 77.9644887724918 52.1556 48.0000 19.9763 186
1024 8.7647 0.0709 L 0.7460 57.7647 56.0000 25.7751 34 6.57952451705933 77.4296249820534 51.3436 49.0000 18.9531 169

Total number of rows: 36227

Table truncated, full table size 4344 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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