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Sample GSM218363 Query DataSets for GSM218363
Status Public on Jul 08, 2008
Title Lethal Yellow mice pioglitazone treated #3
Sample type RNA
 
Source name ovary
Organism Mus musculus
Characteristics Ovary from pioglitazone-treated obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with pioglitazone (0.1 mg/day) from 120 to 180 days of age.
Extracted molecule total RNA
Extraction protocol The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
Label Alexa-647
Label protocol see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
 
Hybridization protocol CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
Scan protocol Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
Description IACUC approval was obtained for all animal treatments.
Data processing global median normalization, CodeLink Expression Analysis 4.1.0.4163
 
Submission date Aug 15, 2007
Last update date Feb 12, 2009
Contact name Kathleen M Eyster
E-mail(s) Kathleen.Eyster@usd.edu
Organization name University of South Dakota
Department Basic Biomedical Sciences
Street address 414 E. Clark St.
City Vermillion
State/province SD
ZIP/Postal code 57069
Country USA
 
Platform ID GPL2897
Series (1)
GSE8806 Pioglitazone treatment of Lethal Yellow mice

Data table header descriptions
ID_REF
Raw_intensity Raw signal intensity value
VALUE Median normalized signal intensity value
Quality_flag CodeLink quality flag
Signal_strength Signal strength
Spot_mean Spot mean
Spot_median Spot median
Spot_stdev Spot standard deviation
Spot_area Spot area
Spot_diameter Spot diameter
Spot_noise_level Spot noise level
Bkgd_mean Background mean
Bkgd_median Background median
Bkgd_stdev Background standard deviation
Bkgd_area Background area

Data table
ID_REF Raw_intensity VALUE Quality_flag Signal_strength Spot_mean Spot_median Spot_stdev Spot_area Spot_diameter Spot_noise_level Bkgd_mean Bkgd_median Bkgd_stdev Bkgd_area
1001 7970.9067 50.4128 G 78.8343 8033.9067 10586.0000 6235.4941 131 12.9148902893066 101.90874648649 65.3782 63.0000 25.9392 123
1002 2609.4233 16.5036 G 25.8023 2671.4233 3070.0000 1468.4851 139 13.303394317627 103.534465578184 65.0177 62.0000 27.6896 234
1003 89.3134 0.5649 G 1.5273 150.3134 145.0000 55.4752 67 9.23618125915527 98.4155692170083 61.8407 61.0000 24.9437 188
1004 158.8447 1.0046 G 2.2874 215.8447 218.0000 70.2113 105 11.562445640564 94.3607372143138 59.8167 57.0000 24.9072 124
1005 149.7714 0.9472 G 2.0825 208.7714 217.0000 64.3264 70 9.44069766998291 100.248788950193 63.9451 59.0000 27.4992 188
1006 14.5263 0.0919 L 0.7441 74.5263 69.0000 33.4817 57 8.51907634735107 100.154264332257 62.4087 60.0000 26.7695 119
1007 7775.8789 49.1794 G 78.9594 7836.8789 9757.0000 6280.2012 134 13.0619335174561 99.2519634285021 65.2417 61.0000 25.5013 124
1008 8194.7168 51.8283 G 86.9442 8257.7168 10302.0000 6474.4111 129 12.8159236907959 94.9771612822269 65.0370 63.0000 21.3181 112
1010 300.1454 1.8983 G 3.9690 354.1454 363.0000 106.6096 112 11.9416427612305 89.2287491522512 58.3750 54.0000 23.4858 124
1012 748.2474 4.7324 G 9.0872 804.2474 879.0000 391.1022 97 11.1132459640503 88.5029542454028 58.0085 56.0000 21.6686 121
1013 82.1475 0.5196 G 1.4545 139.1475 139.0000 51.2032 61 8.81292343139648 95.6668143018859 61.8681 57.0000 25.7779 188
1014 7913.3511 50.0488 G 89.1439 7966.3511 9018.0000 6353.7876 133 13.0131034851074 89.3650743576684 56.9917 53.0000 24.2434 124
1015 8193.7939 51.8225 G 87.6680 8252.7939 8257.0000 6716.1924 143 13.4934520721436 94.1368620616883 59.3909 59.0000 23.4246 114
1017 12.9636 0.0820 L 0.7502 73.9636 66.0000 35.9929 55 8.36828422546387 98.5964245580834 63.8958 61.0000 25.0643 98
1018 67.8966 0.4294 G 1.3295 124.8966 124.0000 45.3122 87 10.5248203277588 93.9439342840572 60.8333 57.0000 24.6293 112
1019 98.3175 0.6218 G 1.6008 160.3175 153.0000 51.6852 63 8.95623207092285 100.14620251892 64.6374 62.0000 25.4308 188
1020 29.8772 0.1890 L 0.8879 85.8772 81.0000 41.8049 57 8.51907634735107 96.7206144824198 62.3423 56.0000 27.1471 115
1021 8089.1113 51.1604 G 92.1884 8146.1113 10029.0000 6422.5977 137 13.2073392868042 88.3637710948976 57.0909 57.0000 20.9092 103
1022 7734.8105 48.9196 G 76.8001 7794.8105 9065.0000 6157.6035 118 12.2573356628418 101.494814054084 63.3833 60.0000 27.6632 124
1024 12.8352 0.0812 L 0.7311 70.8352 70.0000 34.9896 91 10.7640514373779 96.8876464952346 63.1267 58.0000 25.9251 229

Total number of rows: 36227

Table truncated, full table size 4361 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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