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Sample GSM218364 Query DataSets for GSM218364
Status Public on Jul 08, 2008
Title Lethal Yellow mice pioglitazone treated #4
Sample type RNA
 
Source name ovary
Organism Mus musculus
Characteristics Ovary from pioglitazone-treated obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with pioglitazone (0.1 mg/day) from 120 to 180 days of age.
Extracted molecule total RNA
Extraction protocol The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
Label Alexa-647
Label protocol see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
 
Hybridization protocol CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
Scan protocol Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
Description IACUC approval was obtained for all animal treatments.
Data processing global median normalization, CodeLink Expression Analysis 4.1.0.4163
 
Submission date Aug 15, 2007
Last update date Feb 12, 2009
Contact name Kathleen M Eyster
E-mail(s) Kathleen.Eyster@usd.edu
Organization name University of South Dakota
Department Basic Biomedical Sciences
Street address 414 E. Clark St.
City Vermillion
State/province SD
ZIP/Postal code 57069
Country USA
 
Platform ID GPL2897
Series (1)
GSE8806 Pioglitazone treatment of Lethal Yellow mice

Data table header descriptions
ID_REF
Raw_intensity Raw signal intensity value
VALUE Median normalized signal intensity value
Quality_flag CodeLink quality flag
Signal_strength Signal strength
Spot_mean Spot mean
Spot_median Spot median
Spot_stdev Spot standard deviation
Spot_area Spot area
Spot_diameter Spot diameter
Spot_noise_level Spot noise level
Bkgd_mean Background mean
Bkgd_median Background median
Bkgd_stdev Background standard deviation
Bkgd_area Background area

Data table
ID_REF Raw_intensity VALUE Quality_flag Signal_strength Spot_mean Spot_median Spot_stdev Spot_area Spot_diameter Spot_noise_level Bkgd_mean Bkgd_median Bkgd_stdev Bkgd_area
1001 5848.8418 43.6612 G 69.7392 5896.8418 8358.0000 4234.0669 160 14.2729930877686 84.5556866155773 54.4101 48.0000 24.3705 225
1002 1999.3398 14.9249 G 24.0017 2049.3398 2341.0000 1027.9397 155 14.04820728302 85.3830327289459 55.9524 50.0000 23.5887 218
1003 38.3103 0.2860 G 1.0497 85.3103 84.0000 34.3064 87 10.5248203277588 81.2717510807784 54.1008 47.0000 22.8478 123
1004 164.6288 1.2289 G 2.5352 214.6288 200.0000 85.3686 134 13.0619335174561 84.6587076691588 54.9367 50.0000 23.1058 229
1005 156.5976 1.1690 G 2.4115 209.5976 198.0000 68.3855 82 10.2179079055786 86.9176264986494 56.2265 53.0000 22.6117 187
1006 26.5128 0.1979 L 0.8913 76.5128 69.0000 41.4846 39 7.04672574996948 85.8476075168215 52.8817 50.0000 23.8984 175
1007 5986.1841 44.6865 G 70.0045 6036.1841 8331.0000 4137.5288 154 14.0028171539307 86.2256094537326 56.5571 50.0000 24.1504 227
1008 5780.9756 43.1546 G 73.4412 5828.9756 7528.0000 4451.5825 165 14.4942932128906 79.3692771911658 53.4648 48.0000 20.9129 221
1010 259.9712 1.9407 G 3.5046 310.9712 308.0000 110.4445 141 13.3987598419189 88.7319798051772 55.7126 51.0000 25.1547 180
1012 777.7476 5.8058 G 9.9907 826.7476 847.0000 380.8160 105 11.562445640564 82.7517721695521 53.1239 49.0000 22.5012 117
1013 73.1059 0.5457 G 1.4475 124.1059 118.0000 45.0660 85 10.4031419754028 85.7378967838434 56.1356 51.0000 23.1586 122
1014 5557.6548 41.4875 G 72.6258 5604.6548 7152.0000 4462.7822 173 14.8415107727051 77.1716516030949 50.9641 47.0000 20.1144 231
1015 6225.6685 46.4742 G 72.7539 6276.6685 9194.0000 4690.7036 168 14.6254653930664 86.2726606447803 55.1491 51.0000 23.5151 236
1017 21.6667 0.1617 L 0.9219 74.6667 69.0000 34.1486 63 8.95623207092285 80.9882157406156 55.2262 53.0000 18.6588 86
1018 85.4545 0.6379 G 1.7004 133.4545 132.0000 43.6179 112 11.9416427612305 78.4832709730731 50.9750 48.0000 20.3222 124
1019 71.6212 0.5346 G 1.5157 118.6212 110.0000 48.7174 66 9.16699600219727 78.2595798820491 52.7967 47.0000 20.8397 188
1020 40.7632 0.3043 G 1.1108 88.7632 91.0000 34.5885 76 9.83698177337646 79.9080080534807 55.1017 48.0000 21.2720 122
1021 6010.6587 44.8692 G 74.0912 6059.6587 8774.0000 4560.0186 169 14.6689291000366 81.7864729099131 54.8281 49.0000 21.8577 198
1022 6412.7681 47.8709 G 74.7029 6461.7681 8347.0000 4581.1084 140 13.3511619567871 86.4995833101839 55.6750 49.0000 24.9997 124
1024 11.6552 0.0870 L 0.7309 61.6552 59.0000 27.1800 87 10.5248203277588 84.3566648393777 55.3141 50.0000 22.9044 197

Total number of rows: 36227

Table truncated, full table size 4354 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Processed data included within Sample table

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