|
| Status |
Public on Jun 03, 2016 |
| Title |
439551_A01_Sham_1_2010-10-05_532 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse C57BL/6 wild type
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
genotype: wildtype
disease state: Normal
gender: male
age: 8-10 weeks
|
| Treatment protocol |
Oral administration of tamoxifen to wild type and calreticulin transgenic mice to 8-10 weeks-old-mice for 3 weeks was conducted to induce calreticulin expresssion in the heart
|
| Growth protocol |
Wild type and transgenic mice were grown in standard animal facility with 12 hour light/dark cycle, and 8-10 weeks-old-male-mice were used in this study
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from wild type and transgenic heart homogenates using TRIzol reagent (Invitrogen), and a double strand cDNA library was constructed from 300 ng of purified RNA using a library synthesis kit (Sigma)
|
| Label |
Cy3
|
| Label protocol |
Purified cDNA was mixed with Cy3 primer and dNTP/Klenow fragment (50 U/ul) (NimbleGen one color labelling kit), and incubated for 2 h at 37oC with a heated lid
|
| |
|
| Hybridization protocol |
Hybridization to mouse MM9 Expression Array (12 x 135K, NimbleGen) was carried out at 42oC for 20 h in an HX12 Mixer (Roche), to acquire uniform signal and high sensitivity with minimal sample volume across the array surface
|
| Scan protocol |
Arrays were scanned using NimbleGen MS 200 software, and the data were extracted using NimbleScan software
|
| Description |
This sample is of wild-type mouse C57BL/6. It is the first of three wild-type biological replicates used in this experiment
|
| Data processing |
Array normalization was performed using the quantile normalization method of Bolstad et al (Bolstad, Irizarry et al., 2003). n-fold change ratios for a particular gene in calreticulin transgenic and control heart were calculated using the RMA-processed expression values (RMA calls)
|
| |
|
| Submission date |
Jun 02, 2016 |
| Last update date |
Jun 03, 2016 |
| Contact name |
Marek Michalak |
| E-mail(s) |
marek.michalak@ualberta.ca
|
| Phone |
780-492-2256
|
| Organization name |
University of Alberta
|
| Department |
Biochemistry
|
| Lab |
3-55 MSB
|
| Street address |
87 Avenue
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6H 2S7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10192 |
| Series (1) |
| GSE82188 |
Gene expression analysis of cardiac-specific calreticulin transgenic mice |
|
