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Sample GSM2188955 Query DataSets for GSM2188955
Status Public on Jun 01, 2018
Title FT2 Control
Sample type RNA
 
Source name FT2
Organism Mus musculus
Characteristics tissue: Fallopian tube
Treatment protocol Tumors were generated by treatment of mice with doxycycline. Control mice were also treated with doxycycline before fallopian tube extraction.
Growth protocol Mice were bred following the IACUC protocol at MD Anderson Cancer Center mouse facility.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from fallopian tube of wild type mice treated with doxycycline
Data processing Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
 
Submission date Jun 06, 2016
Last update date Jun 01, 2018
Contact name Sharmistha Sarkar
Organization name The University of Texas MD Anderson Cancer Center
Department Genomic Medicine
Street address 1515 Holcombe Boulevard
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL16368
Series (1)
GSE82319 PRKCI promotes immune suppression in ovarian cancer through YAP1

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
100008567_at 6.880217606
100009600_at 4.910512711
100012_at 4.403255399
100017_at 6.093068458
100019_at 6.70787934
100034251_at 10.80531246
100034739_at 3.619389018
100034748_at 3.636979246
100036520_at 3.428295225
100036521_at 8.35899513
100036523_at 3.638084431
100036768_at 3.34410836
100037258_at 8.333558776
100037262_at 4.509826568
100037278_at 5.663007712
100038347_at 7.62292005
100038358_at 3.507567669
100038371_at 2.663247028
100038392_at 3.595013866
100038398_at 5.416149531

Total number of rows: 17566

Table truncated, full table size 365 Kbytes.




Supplementary file Size Download File type/resource
GSM2188955_05_FT2.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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