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Sample GSM2188957 Query DataSets for GSM2188957
Status Public on Jun 01, 2018
Title 291 Tumor
Sample type RNA
Source name Tumor 291
Organism Mus musculus
Characteristics tissue: Tumor from GEMM
Treatment protocol Tumors were generated by treatment of mice with doxycycline. Control mice were also treated with doxycycline before fallopian tube extraction.
Growth protocol Mice were bred following the IACUC protocol at MD Anderson Cancer Center mouse facility.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from tumors of doxycycline inducible GEMM
Data processing Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
Submission date Jun 06, 2016
Last update date Jun 01, 2018
Contact name Sharmistha Sarkar
Organization name The University of Texas MD Anderson Cancer Center
Department Genomic Medicine
Street address 1515 Holcombe Boulevard
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platform ID GPL16368
Series (1)
GSE82319 PRKCI promotes immune suppression in ovarian cancer through YAP1

Data table header descriptions
VALUE normalized signal

Data table
100008567_at 6.658028209
100009600_at 5.414733379
100012_at 2.748352489
100017_at 7.686329673
100019_at 7.631063557
100034251_at 10.86912601
100034739_at 3.882294039
100034748_at 4.084075285
100036520_at 3.36304267
100036521_at 7.194410245
100036523_at 3.960988535
100036768_at 3.760612082
100037258_at 7.836590007
100037262_at 4.474851911
100037278_at 6.32605657
100038347_at 5.820830391
100038358_at 3.296553999
100038371_at 2.905453308
100038392_at 3.758167488
100038398_at 3.68014484

Total number of rows: 17566

Table truncated, full table size 365 Kbytes.

Supplementary file Size Download File type/resource
GSM2188957_07_291.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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