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Sample GSM2188958 Query DataSets for GSM2188958
Status Public on Jun 01, 2018
Title 316 Tumor
Sample type RNA
 
Source name Tumor 316
Organism Mus musculus
Characteristics tissue: Tumor from GEMM
Treatment protocol Tumors were generated by treatment of mice with doxycycline. Control mice were also treated with doxycycline before fallopian tube extraction.
Growth protocol Mice were bred following the IACUC protocol at MD Anderson Cancer Center mouse facility.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from tumors of doxycycline inducible GEMM
Data processing Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
 
Submission date Jun 06, 2016
Last update date Jun 01, 2018
Contact name Sharmistha Sarkar
Organization name The University of Texas MD Anderson Cancer Center
Department Genomic Medicine
Street address 1515 Holcombe Boulevard
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL16368
Series (1)
GSE82319 PRKCI promotes immune suppression in ovarian cancer through YAP1

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
100008567_at 6.252078177
100009600_at 4.922865014
100012_at 2.658014986
100017_at 7.610317593
100019_at 7.445597023
100034251_at 9.26557425
100034739_at 3.978867522
100034748_at 3.815607724
100036520_at 3.817952524
100036521_at 8.519000278
100036523_at 3.69490466
100036768_at 3.434902197
100037258_at 8.2030748
100037262_at 4.415392745
100037278_at 5.883612527
100038347_at 7.539855228
100038358_at 3.431544253
100038371_at 2.707566606
100038392_at 3.601209062
100038398_at 4.627209332

Total number of rows: 17566

Table truncated, full table size 365 Kbytes.




Supplementary file Size Download File type/resource
GSM2188958_08_316.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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