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Sample GSM2193206 Query DataSets for GSM2193206
Status Public on Jul 21, 2016
Title CD4+_T_cells_COH
Sample type SRA
 
Source name Cells
Organism Homo sapiens
Characteristics tissue type: CD4+ T cells
donor: Human (donor B)
Treatment protocol All the samples were histologically confirmed to be normal
Growth protocol Donor E is a fetus, donor A and B were adults
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tissue by using the miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiScanSQ
 
Description Illumina HiScanSQ (COH)
Data processing Trim low quality reads and adapters using Trimmomatic software
Mapping trimmed reads to human reference genome using TopHat software
Transcript assembly and quantification using Cufflinks software
Genome_build: GRCh37
Supplementary_files_format_and_content: Table-delimited text files include FPKM value of 156600 transcripts across 28 samples
 
Submission date Jun 08, 2016
Last update date May 15, 2019
Contact name Leming Shi
E-mail(s) lemingshi@fudan.edu.cn
Phone +86-18616827008
Organization name Fudan University
Department School of Life Sciences
Lab Center for Pharmacogenomics
Street address 2005 Songhu Road
City Shanghai
ZIP/Postal code 200438
Country China
 
Platform ID GPL15456
Series (1)
GSE83115 Identification of Tissue-Specific Protein-Coding and Noncoding Transcripts across 14 Human Tissues Using RNA-seq
Relations
BioSample SAMN05214514
SRA SRX1830408

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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