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Sample GSM219506 Query DataSets for GSM219506
Status Public on Aug 22, 2007
Title CLL17-CD8
Sample type RNA
 
Source name peripheral blood CD8 T cells
Organism Homo sapiens
Characteristics CLL
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix hu133A microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description peripheral blood CD8 T cells: CLL
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Sep 01, 2016
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL96
Series (1)
GSE8835 Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells.
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 167.5 P 0.000857
AFFX-BioB-M_at 408.2 P 0.000044
AFFX-BioB-3_at 255.3 P 0.000044
AFFX-BioC-5_at 443.4 P 0.00007
AFFX-BioC-3_at 389.7 P 0.000044
AFFX-BioDn-5_at 474.1 P 0.000044
AFFX-BioDn-3_at 1931.3 P 0.000044
AFFX-CreX-5_at 3795.5 P 0.000044
AFFX-CreX-3_at 5359.8 P 0.000044
AFFX-DapX-5_at 0.9 A 0.659352
AFFX-DapX-M_at 8 A 0.368438
AFFX-DapX-3_at 2.5 A 0.834139
AFFX-LysX-5_at 0.8 A 0.852061
AFFX-LysX-M_at 2.9 A 0.574038
AFFX-LysX-3_at 6.2 A 0.250796
AFFX-PheX-5_at 0.4 A 0.969024
AFFX-PheX-M_at 0.8 A 0.932322
AFFX-PheX-3_at 4.5 A 0.470241
AFFX-ThrX-5_at 0.9 A 0.916408
AFFX-ThrX-M_at 1 A 0.860518

Total number of rows: 22283

Table truncated, full table size 575 Kbytes.




Supplementary file Size Download File type/resource
GSM219506.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM219506.TXT.gz 227.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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