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Sample GSM219526 Query DataSets for GSM219526
Status Public on Dec 01, 2007
Title CLL1-CD4
Sample type RNA
Source name splenic CD4 T cells
Organism Mus musculus
Characteristics Em-TCL1,13.5mo old, Female, CLL
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD4 T cells_Em-TCL1,13.5mo old, Female, CLL
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 523 P 0.000044
AFFX-BioB-M_at 995.6 P 0.000044
AFFX-BioB-3_at 813 P 0.000044
AFFX-BioC-5_at 1888.1 P 0.000044
AFFX-BioC-3_at 1298.9 P 0.000044
AFFX-BioDn-5_at 3330.3 P 0.000044
AFFX-BioDn-3_at 5926.4 P 0.000044
AFFX-CreX-5_at 9440.7 P 0.000052
AFFX-CreX-3_at 10124.8 P 0.000044
AFFX-DapX-5_at 0.3 A 0.941572
AFFX-DapX-M_at 2.8 A 0.544587
AFFX-DapX-3_at 0.5 A 0.910522
AFFX-LysX-5_at 1.7 A 0.485109
AFFX-LysX-M_at 0.4 A 0.852061
AFFX-LysX-3_at 0.6 A 0.51489
AFFX-PheX-5_at 0.5 A 0.834139
AFFX-PheX-M_at 0.2 A 0.966335
AFFX-PheX-3_at 1.3 A 0.699457
AFFX-ThrX-5_at 0.9 A 0.645547
AFFX-ThrX-M_at 3 A 0.382589

Total number of rows: 45101

Table truncated, full table size 1158 Kbytes.

Supplementary file Size Download File type/resource
GSM219526.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM219526.TXT.gz 443.6 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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