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Sample GSM219528 Query DataSets for GSM219528
Status Public on Dec 01, 2007
Title CLL3-CD4
Sample type RNA
Source name splenic CD4 T cells
Organism Mus musculus
Characteristics EmTCL1, 17mo old, Male, CLL
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD4 T cells_EmTCL1, 17mo old, Male, CLL
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 362.5 P 0.000509
AFFX-BioB-M_at 636.6 P 0.000044
AFFX-BioB-3_at 398.6 P 0.000044
AFFX-BioC-5_at 1377.5 P 0.000052
AFFX-BioC-3_at 1336.1 P 0.000044
AFFX-BioDn-5_at 2076 P 0.000044
AFFX-BioDn-3_at 5278 P 0.000052
AFFX-CreX-5_at 8874.2 P 0.000052
AFFX-CreX-3_at 10201.9 P 0.000044
AFFX-DapX-5_at 4.7 A 0.382599
AFFX-DapX-M_at 7.5 A 0.617401
AFFX-DapX-3_at 2.5 A 0.659339
AFFX-LysX-5_at 0.7 A 0.986189
AFFX-LysX-M_at 1.7 A 0.916408
AFFX-LysX-3_at 4.1 A 0.455409
AFFX-PheX-5_at 0.7 A 0.883887
AFFX-PheX-M_at 0.9 A 0.949771
AFFX-PheX-3_at 8.7 A 0.354453
AFFX-ThrX-5_at 4 A 0.749204
AFFX-ThrX-M_at 0.6 A 0.891041

Total number of rows: 45101

Table truncated, full table size 1190 Kbytes.

Supplementary file Size Download File type/resource
GSM219528.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM219528.TXT.gz 458.0 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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