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Sample GSM219541 Query DataSets for GSM219541
Status Public on Dec 01, 2007
Title Healthy4-CD4
Sample type RNA
 
Source name splenic CD4 T cells
Organism Mus musculus
Characteristics Em-Tcl1, 9mo old, Male, Healthy
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD4 T cells_Em-Tcl1, 9mo old, Male, Healthy
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 308.2 P 0.000754
AFFX-BioB-M_at 716.8 P 0.000044
AFFX-BioB-3_at 332.9 P 0.000044
AFFX-BioC-5_at 926.1 P 0.000044
AFFX-BioC-3_at 758.2 P 0.000044
AFFX-BioDn-5_at 1018.4 P 0.000044
AFFX-BioDn-3_at 4035.5 P 0.000044
AFFX-CreX-5_at 11165.9 P 0.000052
AFFX-CreX-3_at 11336.9 P 0.000044
AFFX-DapX-5_at 4.7 A 0.699394
AFFX-DapX-M_at 9.4 A 0.327065
AFFX-DapX-3_at 0.8 A 0.990663
AFFX-LysX-5_at 1.4 A 0.559354
AFFX-LysX-M_at 10.3 A 0.51489
AFFX-LysX-3_at 3.8 A 0.455413
AFFX-PheX-5_at 1.4 A 0.814953
AFFX-PheX-M_at 0.7 A 0.966323
AFFX-PheX-3_at 5.1 A 0.544587
AFFX-ThrX-5_at 8.5 A 0.41138
AFFX-ThrX-M_at 3.9 A 0.559354

Total number of rows: 45101

Table truncated, full table size 1193 Kbytes.




Supplementary file Size Download File type/resource
GSM219541.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM219541.TXT.gz 460.7 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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