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Sample GSM219547 Query DataSets for GSM219547
Status Public on Dec 01, 2007
Title IV-CLL4-CD4
Sample type RNA
 
Source name splenic CD4 T cells
Organism Mus musculus
Characteristics Em-Tcl1, 3mo old, Male
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD4 T cells_Em-Tcl1, 3mo old, Male
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 247.5 P 0.000509
AFFX-BioB-M_at 390.7 P 0.000044
AFFX-BioB-3_at 233.8 P 0.000044
AFFX-BioC-5_at 818.7 P 0.000052
AFFX-BioC-3_at 928.2 P 0.000044
AFFX-BioDn-5_at 1643.9 P 0.000044
AFFX-BioDn-3_at 3595.6 P 0.000052
AFFX-CreX-5_at 7644.2 P 0.000052
AFFX-CreX-3_at 8142.7 P 0.000044
AFFX-DapX-5_at 8.2 A 0.139482
AFFX-DapX-M_at 8.4 A 0.440646
AFFX-DapX-3_at 0.8 A 0.99238
AFFX-LysX-5_at 1.5 A 0.51489
AFFX-LysX-M_at 12.4 A 0.544587
AFFX-LysX-3_at 12.2 P 0.004017
AFFX-PheX-5_at 1.2 A 0.814869
AFFX-PheX-M_at 0.7 A 0.91056
AFFX-PheX-3_at 3.8 A 0.794268
AFFX-ThrX-5_at 6.5 A 0.354453
AFFX-ThrX-M_at 7.2 A 0.425962

Total number of rows: 45101

Table truncated, full table size 1194 Kbytes.




Supplementary file Size Download File type/resource
GSM219547.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM219547.TXT.gz 459.9 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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