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Sample GSM219549 Query DataSets for GSM219549
Status Public on Dec 01, 2007
Title IV-CLL6-CD4
Sample type RNA
Source name splenic CD4 T cells
Organism Mus musculus
Characteristics Em-Tcl1, 3mo old, Male
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD4 T cells_Em-Tcl1, 3mo old, Male
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 257.7 P 0.000297
AFFX-BioB-M_at 452.1 P 0.000044
AFFX-BioB-3_at 277.9 P 0.000044
AFFX-BioC-5_at 944.6 P 0.000052
AFFX-BioC-3_at 1045.9 P 0.000044
AFFX-BioDn-5_at 1771.3 P 0.000044
AFFX-BioDn-3_at 4196.1 P 0.00007
AFFX-CreX-5_at 8310.9 P 0.000052
AFFX-CreX-3_at 8926.4 P 0.000044
AFFX-DapX-5_at 8.9 A 0.216504
AFFX-DapX-M_at 6.3 A 0.327079
AFFX-DapX-3_at 0.7 A 0.989683
AFFX-LysX-5_at 4 A 0.455413
AFFX-LysX-M_at 2 A 0.860518
AFFX-LysX-3_at 1.2 A 0.574038
AFFX-PheX-5_at 0.6 A 0.957038
AFFX-PheX-M_at 1.9 A 0.48511
AFFX-PheX-3_at 5 A 0.52976
AFFX-ThrX-5_at 15.6 A 0.39692
AFFX-ThrX-M_at 0.5 A 0.783476

Total number of rows: 45101

Table truncated, full table size 1189 Kbytes.

Supplementary file Size Download File type/resource
GSM219549.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM219549.TXT.gz 457.6 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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