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Sample GSM219550 Query DataSets for GSM219550
Status Public on Dec 01, 2007
Title WT1-CD8
Sample type RNA
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics B6C3, 5mo old, Male
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_B6C3, 5mo old, Male
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 259 P 0.00006
AFFX-BioB-M_at 381.2 P 0.000044
AFFX-BioB-3_at 265.7 P 0.000044
AFFX-BioC-5_at 879.9 P 0.000052
AFFX-BioC-3_at 900.6 P 0.000044
AFFX-BioDn-5_at 1472 P 0.000044
AFFX-BioDn-3_at 3736.2 P 0.000052
AFFX-CreX-5_at 7670.3 P 0.000044
AFFX-CreX-3_at 7550.2 P 0.000044
AFFX-DapX-5_at 1.7 A 0.824672
AFFX-DapX-M_at 11.9 A 0.340661
AFFX-DapX-3_at 2.4 A 0.86866
AFFX-LysX-5_at 5.1 A 0.672921
AFFX-LysX-M_at 2.4 A 0.834139
AFFX-LysX-3_at 1.9 A 0.916408
AFFX-PheX-5_at 1.4 A 0.949771
AFFX-PheX-M_at 2.3 A 0.712257
AFFX-PheX-3_at 4 A 0.772364
AFFX-ThrX-5_at 4.8 A 0.760937
AFFX-ThrX-M_at 4.7 A 0.559354

Total number of rows: 45101

Table truncated, full table size 1188 Kbytes.

Supplementary file Size Download File type/resource
GSM219550.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM219550.TXT.gz 458.1 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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