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Sample GSM219551 Query DataSets for GSM219551
Status Public on Dec 01, 2007
Title WT2-CD8
Sample type RNA
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics B6C3, 5mo old, Female
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_B6C3, 5mo old, Female
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 356.3 P 0.000095
AFFX-BioB-M_at 571.8 P 0.000044
AFFX-BioB-3_at 324.3 P 0.000044
AFFX-BioC-5_at 1127 P 0.000052
AFFX-BioC-3_at 1243.9 P 0.000044
AFFX-BioDn-5_at 2111.1 P 0.000044
AFFX-BioDn-3_at 5022.5 P 0.000044
AFFX-CreX-5_at 9214.9 P 0.000052
AFFX-CreX-3_at 9911.1 P 0.000044
AFFX-DapX-5_at 8 A 0.185131
AFFX-DapX-M_at 5.5 A 0.749204
AFFX-DapX-3_at 2.1 A 0.916408
AFFX-LysX-5_at 1.4 A 0.712274
AFFX-LysX-M_at 2.6 A 0.783476
AFFX-LysX-3_at 9.9 A 0.340661
AFFX-PheX-5_at 0.7 A 0.995002
AFFX-PheX-M_at 0.8 A 0.957038
AFFX-PheX-3_at 2.8 A 0.783476
AFFX-ThrX-5_at 12.1 A 0.147855
AFFX-ThrX-M_at 1.3 A 0.883887

Total number of rows: 45101

Table truncated, full table size 1191 Kbytes.

Supplementary file Size Download File type/resource
GSM219551.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM219551.TXT.gz 458.1 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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