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Sample GSM219552 Query DataSets for GSM219552
Status Public on Dec 01, 2007
Title WT3-CD8
Sample type RNA
 
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics B6C3, 5mo old, Male
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_B6C3, 5mo old, Male
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 286.4 P 0.000127
AFFX-BioB-M_at 524.4 P 0.000044
AFFX-BioB-3_at 382.5 P 0.000044
AFFX-BioC-5_at 1071.5 P 0.000044
AFFX-BioC-3_at 950.1 P 0.000044
AFFX-BioDn-5_at 1798.9 P 0.000044
AFFX-BioDn-3_at 4212.1 P 0.000044
AFFX-CreX-5_at 8011.8 P 0.000052
AFFX-CreX-3_at 8571.6 P 0.000044
AFFX-DapX-5_at 7.4 A 0.327079
AFFX-DapX-M_at 4.5 A 0.631573
AFFX-DapX-3_at 1.2 A 0.804754
AFFX-LysX-5_at 7.7 A 0.216524
AFFX-LysX-M_at 2 A 0.904333
AFFX-LysX-3_at 10.7 A 0.131361
AFFX-PheX-5_at 1.4 A 0.824672
AFFX-PheX-M_at 1 A 0.978098
AFFX-PheX-3_at 2.6 A 0.860518
AFFX-ThrX-5_at 1.9 A 0.916408
AFFX-ThrX-M_at 1.5 A 0.699394

Total number of rows: 45101

Table truncated, full table size 1185 Kbytes.




Supplementary file Size Download File type/resource
GSM219552.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM219552.TXT.gz 457.0 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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