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Sample GSM219563 Query DataSets for GSM219563
Status Public on Dec 01, 2007
Title WT5-CD8
Sample type RNA
 
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics B6C3, 8.5mo old, Female
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_B6C3, 8.5mo old, Female
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 287.1 P 0.000446
AFFX-BioB-M_at 615.6 P 0.000044
AFFX-BioB-3_at 319.3 P 0.000044
AFFX-BioC-5_at 818.4 P 0.00006
AFFX-BioC-3_at 683.7 P 0.000044
AFFX-BioDn-5_at 943.9 P 0.000044
AFFX-BioDn-3_at 3787 P 0.000044
AFFX-CreX-5_at 10456.5 P 0.000044
AFFX-CreX-3_at 11579.1 P 0.000044
AFFX-DapX-5_at 3.2 A 0.699394
AFFX-DapX-M_at 18.6 A 0.131361
AFFX-DapX-3_at 1.6 A 0.834139
AFFX-LysX-5_at 1.3 A 0.868639
AFFX-LysX-M_at 3.5 A 0.804734
AFFX-LysX-3_at 10.8 A 0.156732
AFFX-PheX-5_at 9.1 A 0.340661
AFFX-PheX-M_at 4.1 A 0.52976
AFFX-PheX-3_at 12.2 A 0.39692
AFFX-ThrX-5_at 7.9 A 0.699394
AFFX-ThrX-M_at 1.3 A 0.659339

Total number of rows: 45101

Table truncated, full table size 1190 Kbytes.




Supplementary file Size Download File type/resource
GSM219563.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM219563.TXT.gz 459.1 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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