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Sample GSM219566 Query DataSets for GSM219566
Status Public on Dec 01, 2007
Title CLL10-CD8
Sample type RNA
 
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics Em-TCL1, 12mo old, Female, CLL
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_Em-TCL1, 12mo old, Female, CLL
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
 
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dCHIP
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 274.1 P 0.000754
AFFX-BioB-M_at 551.1 P 0.000044
AFFX-BioB-3_at 264.8 P 0.000044
AFFX-BioC-5_at 703.7 P 0.00007
AFFX-BioC-3_at 654.4 P 0.000044
AFFX-BioDn-5_at 776.5 P 0.000044
AFFX-BioDn-3_at 3605.9 P 0.000052
AFFX-CreX-5_at 9572.6 P 0.000052
AFFX-CreX-3_at 10788.8 P 0.000044
AFFX-DapX-5_at 6.4 A 0.52976
AFFX-DapX-M_at 1.1 A 0.783476
AFFX-DapX-3_at 7.7 A 0.470241
AFFX-LysX-5_at 9 A 0.156732
AFFX-LysX-M_at 2.5 A 0.910522
AFFX-LysX-3_at 12.5 P 0.001796
AFFX-PheX-5_at 1.2 A 0.814869
AFFX-PheX-M_at 1 A 0.973889
AFFX-PheX-3_at 16.3 A 0.216524
AFFX-ThrX-5_at 3.1 A 0.574038
AFFX-ThrX-M_at 1 A 0.834139

Total number of rows: 45101

Table truncated, full table size 1189 Kbytes.




Supplementary file Size Download File type/resource
GSM219566.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM219566.TXT.gz 458.2 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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