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Sample GSM219570 Query DataSets for GSM219570
Status Public on Dec 01, 2007
Title CLL11-CD8
Sample type RNA
Source name splenic CD8 T cells
Organism Mus musculus
Characteristics Em-TCL1, 12mo old, Male, CLL
Treatment protocol CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
Growth protocol T cells were isolated from fresh tissues or peripheral blood.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix moe430_2.0 microarray chip.
Scan protocol Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
Description splenic CD8 T cells_Em-TCL1, 12mo old, Male, CLL
Data processing The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
Submission date Aug 21, 2007
Last update date Aug 28, 2018
Contact name Gullu Gorgun
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE8836 CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Reanalyzed by GSE119085

Data table header descriptions

Data table
AFFX-BioB-5_at 347.6 P 0.000446
AFFX-BioB-M_at 773.5 P 0.000044
AFFX-BioB-3_at 378.6 P 0.000044
AFFX-BioC-5_at 885.5 P 0.00006
AFFX-BioC-3_at 840.7 P 0.000044
AFFX-BioDn-5_at 1017.1 P 0.000044
AFFX-BioDn-3_at 4478.3 P 0.000044
AFFX-CreX-5_at 11580.8 P 0.000052
AFFX-CreX-3_at 12778.4 P 0.000044
AFFX-DapX-5_at 8.6 A 0.116092
AFFX-DapX-M_at 9.1 A 0.354453
AFFX-DapX-3_at 1.4 A 0.973889
AFFX-LysX-5_at 1.4 A 0.672935
AFFX-LysX-M_at 1.5 A 0.921998
AFFX-LysX-3_at 9.2 A 0.116113
AFFX-PheX-5_at 0.7 A 0.979978
AFFX-PheX-M_at 1.8 A 0.824672
AFFX-PheX-3_at 7 A 0.440646
AFFX-ThrX-5_at 13.8 A 0.39692
AFFX-ThrX-M_at 10.4 A 0.18511

Total number of rows: 45101

Table truncated, full table size 1192 Kbytes.

Supplementary file Size Download File type/resource
GSM219570.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM219570.TXT.gz 460.4 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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