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Sample GSM2199346 Query DataSets for GSM2199346
Status Public on Aug 01, 2016
Title MEF-V, biological rep1
Sample type RNA
 
Source name Mouse embryonic fibroblasts expressing empty expression vector (MEF-V)
Organism Mus musculus
Characteristics tissue: embryonic fibroblasts
cell line: SV40-immortalized (ATCC, CRL-2907)
vector: empty expression vector (MEF-V)
Treatment protocol Cells were harvested by trypsinization
Growth protocol SV40-immortalized mouse embryonic fibroblast (ATCC) cell lines were maintained in Dulbecco's modified Eagle's medium, supplemented with 10% fetal calf serum, at 37˚C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
 
Hybridization protocol Biotininylated probes were hybridized to Affymetrix Mouse Gene 1.0 ST arrays using Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from mouse embryonic fibroblasts
Data processing Data were analyzed by BRB Array Tools, GeneSpring GX 12.6 (Agilent Technologies) and Partek Genomics Suite 6.5 software
 
Submission date Jun 13, 2016
Last update date Aug 01, 2016
Contact name Yuri Postnikov
E-mail(s) PostnikY@mail.nih.gov
Phone 301-496-2885
Organization name NIH
Street address 37 Convent Dr
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10740
Series (1)
GSE83313 Expression data from human HMGN4- and HMGN2-expressing mouse embryionic fibroblasts

Data table header descriptions
ID_REF
VALUE Quantification
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
10338001 11.2833 0
10338002 7.11662 0.199832
10338003 9.54703 0
10338004 8.31846 1.31697e-14
10338005 3.79529 0.979005
10338006 4.00747 0.561061
10338007 4.30595 0.944515
10338008 4.8297 0.927434
10338009 8.7177 0.534978
10338010 3.83723 0.182706
10338011 6.67522 0.584613
10338012 3.92368 0.306462
10338013 3.69546 0.340778
10338014 3.73941 0.648845
10338015 3.72896 0.117154
10338016 8.46428 0.635599
10338017 12.7687 3.92184e-36
10338018 7.64066 0.634071
10338019 6.03972 0.269533
10338020 8.89973 0.963361

Total number of rows: 241576

Table truncated, full table size 6416 Kbytes.




Supplementary file Size Download File type/resource
GSM2199346_MEF_vector_1_MoGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM2199346_MEF_vector_1_MoGene-1_0-st-v1_.rma-exon-default-dabg.chp.gz 2.2 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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