mRNA amplification and labeling using a T7 global amplification method (Two-cycle Target Labeling kit; Affymetrix)
Hybridization protocol
Affymetrix Hybridization
Scan protocol
GeneChip Scanner
Description
For tumors: TG1 means transplantation generation 1. H means high, L means low. Therefore, CD29HCD24H means CD29 high, CD24 high; CD29HCD24L means CD29 high, CD24 low; CD29LCD24H means CD29 low, CD24 high; CD29LCD24L means CD29 low, CD24 low.
For normal: MEC means mammary epithelial cell. CD29highCD24positive from normal MEC is shortened to CD29HCD24P(MEC), CD29positiveCD24high from normal is shortened to CD29PCD24H(MEC), and CD24low from normal is shortened to CD24L(MEC).
