|
| Status |
Public on Dec 01, 2007 |
| Title |
Liver_C_week4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, control
|
| Organism |
Mus musculus |
| Characteristics |
C57/BL6J
Female mice
|
| Biomaterial provider |
Animal Holding Unit, National University Singapore
|
| Treatment protocol |
Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner G2500A
|
| Description |
Liver tissue from C57BL/6J mice fed on control diet
|
| Data processing |
The GeneChip Operating Software Version 1.2.0.037 (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
|
| |
|
| Submission date |
Aug 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mei Yen Chan |
| E-mail(s) |
m_y_chan3@hotmail.com
|
| Organization name |
Yong Loo Lin School of Medicine, National University of Singapore
|
| Department |
Pediatrics
|
| Lab |
Cardiovascular Genetics
|
| Street address |
5 Lower Kent Ridge Road
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
119074 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8753 |
Sequential responses to high-fat feeding in an obese mouse model |
|
| Relations |
| Reanalyzed by |
GSE119085 |
