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Sample GSM224695 Query DataSets for GSM224695
Status Public on Dec 01, 2007
Title Liver_C_week4
Sample type RNA
 
Source name Liver, control
Organism Mus musculus
Characteristics C57/BL6J
Female mice
Biomaterial provider Animal Holding Unit, National University Singapore
Treatment protocol Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80┬░C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
 
Hybridization protocol Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner G2500A
Description Liver tissue from C57BL/6J mice fed on control diet
Data processing The GeneChip Operating Software Version 1.2.0.037 (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
 
Submission date Aug 24, 2007
Last update date Aug 28, 2018
Contact name Mei Yen Chan
E-mail(s) m_y_chan3@hotmail.com
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE8753 Sequential responses to high-fat feeding in an obese mouse model
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE signal
ABS_CALL detection
DETECTION P-VALUE detection p value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 592.5 P 0.000754
AFFX-BioB-M_at 1037.4 P 0.001248
AFFX-BioB-3_at 755.3 P 0.000127
AFFX-BioC-5_at 2565.1 P 0.000052
AFFX-BioC-3_at 2103.8 P 0.000052
AFFX-BioDn-5_at 4300.3 P 0.000044
AFFX-BioDn-3_at 7857.6 P 0.000044
AFFX-CreX-5_at 20935.8 P 0.000044
AFFX-CreX-3_at 32158.6 P 0.000044
AFFX-DapX-5_at 7.6 A 0.588627
AFFX-DapX-M_at 45.9 A 0.659339
AFFX-DapX-3_at 16.2 A 0.963431
AFFX-LysX-5_at 49 A 0.41138
AFFX-LysX-M_at 40.3 A 0.852061
AFFX-LysX-3_at 53.2 A 0.425962
AFFX-PheX-5_at 73.5 A 0.41138
AFFX-PheX-M_at 8.4 A 0.910522
AFFX-PheX-3_at 30 A 0.852061
AFFX-ThrX-5_at 91.5 A 0.368438
AFFX-ThrX-M_at 16.3 A 0.794268

Total number of rows: 45101

Table truncated, full table size 1215 Kbytes.




Supplementary file Size Download File type/resource
GSM224695_C4wkliverRealgenechip.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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