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Sample GSM224706 Query DataSets for GSM224706
Status Public on Dec 01, 2007
Title Liver_C_week10
Sample type RNA
 
Source name Liver, control
Organism Mus musculus
Characteristics C57/BL6J
Female mice
Biomaterial provider Animal Holding Unit, National University Singapore
Treatment protocol Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80┬░C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
 
Hybridization protocol Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner G2500A
Description Liver tissue from C57BL/6J mice fed on control diet
Data processing The GeneChip Operating Software Version 1.2.0.037 (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
 
Submission date Aug 24, 2007
Last update date Dec 29, 2011
Contact name Mei Yen Chan
E-mail(s) m_y_chan3@hotmail.com
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
 
Platform ID GPL339
Series (1)
GSE8753 Sequential responses to high-fat feeding in an obese mouse model

Data table header descriptions
ID_REF
VALUE signal
ABS_CALL detection
DETECTION P-VALUE detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1758.4 P 0.000195
AFFX-BioB-M_at 3630.7 P 0.000095
AFFX-BioB-3_at 966.3 P 0.000044
AFFX-BioC-5_at 4186.7 P 0.00007
AFFX-BioC-3_at 3496.3 P 0.000052
AFFX-BioDn-5_at 4080.6 P 0.000044
AFFX-BioDn-3_at 19735.6 P 0.000052
AFFX-CreX-5_at 37292.5 P 0.000044
AFFX-CreX-3_at 49385 P 0.000044
AFFX-DapX-5_at 3 A 0.470241
AFFX-DapX-M_at 8.5 A 0.52976
AFFX-DapX-3_at 8.3 A 0.969024
AFFX-LysX-5_at 47.7 A 0.327079
AFFX-LysX-M_at 10 A 0.891021
AFFX-LysX-3_at 59.3 A 0.300606
AFFX-PheX-5_at 14.5 A 0.724854
AFFX-PheX-M_at 11.2 A 0.724854
AFFX-PheX-3_at 19.8 A 0.724854
AFFX-ThrX-5_at 13.4 A 0.724854
AFFX-ThrX-M_at 94.9 A 0.300606

Total number of rows: 22690

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM224706_control_03.10.03.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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