|
Status |
Public on Sep 01, 2007 |
Title |
Susceptible Group, Ventral tegmental area, Biological Replicate 2 |
Sample type |
RNA |
|
|
Source name |
Mouse Ventral tegmental area
|
Organism |
Mus musculus |
Characteristics |
Midline, 1mm thick, 14 gauge punches, pooled together from four mice (4 total)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with RNAeasy Microkit according to manufacturer protocols
|
Label |
Biotin
|
Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 200ng of total RNA; IVT: 2h
|
|
|
Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 16h at 55 degC
|
Scan protocol |
l Using an Illumina scanner; Standard scan factor.
|
Description |
Ventral tegmental area, male 10.5 week old c57bl/6J mouse, SUSCEPTIBLE group
|
Data processing |
The Raw data was exact using MS5, and Quantile-Quantile Normalized with Affy R package.
|
|
|
Submission date |
Aug 24, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
guanghua xiao |
E-mail(s) |
guanghua.xiao@utsouthwestern.edu
|
Phone |
(214)6484553
|
Organization name |
University of Texas Southwestern Medical Center
|
Department |
Psychiatry
|
Lab |
Eric Nestler
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL4234 |
Series (1) |
GSE8870 |
Global Patterns of Gene Expression Associated with Vulnerability and Resilience to Social Defeat |
|