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Sample GSM224793 Query DataSets for GSM224793
Status Public on Aug 04, 2008
Title WA_52hpf_rep1 (WA52_2-1)
Sample type RNA
 
Source name Wild type whole embryo, 52hpf, pool of ten embryos
Organism Danio rerio
Characteristics Strain: AB
Genotype: Wild-type
Age: 52hpf
Tissue: Whole embryo
Biomaterial provider Yuk Fai Leung/John E. Dowling
Treatment protocol Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate.
Growth protocol Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
Label Biotin
Label protocol Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Wild type whole embryos, 52hpf, pool of ten embryos, biological replicate 1
Data processing The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
 
Submission date Aug 24, 2007
Last update date Aug 14, 2011
Contact name Yuk Fai Leung
E-mail(s) yfleung@purdue.edu
Phone 765-496-3153
Organization name Purdue University
Department Biological Sciences
Lab Leung
Street address 915 W. State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL1319
Series (1)
GSE8874 Factorial Microarray Analysis of Zebrafish Retinal Development

Data table header descriptions
ID_REF
VALUE The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.

Data table
ID_REF VALUE
AFFX-BioB-3_at 391.427
AFFX-BioB-5_at 566.193
AFFX-BioB-M_at 535.033
AFFX-BioC-3_at 1358.76
AFFX-BioC-5_at 1191.81
AFFX-BioDn-3_at 4017.95
AFFX-BioDn-5_at 2315.25
AFFX-CreX-3_at 10186.4
AFFX-CreX-5_at 9098.83
AFFX-DapX-3_at 175.845
AFFX-DapX-5_at 57.2614
AFFX-DapX-M_at 127.743
AFFX-Dr-AB076373-1_at 25.9113
AFFX-Dr-acta1-3_at 16340.3
AFFX-Dr-acta1-5_at 83.3543
AFFX-Dr-acta1-5_x_at 4457.12
AFFX-Dr-acta1-M_at 12317.5
AFFX-Dr-AF292559-1_at 35.7539
AFFX-Dr-AF292559-2_s_at 21.4203
AFFX-Dr-AF292559-3_s_at 0

Total number of rows: 15617

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM224793.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM224793.EXP.gz 519 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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