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Sample GSM224795 Query DataSets for GSM224795
Status Public on Aug 04, 2008
Title WA_52hpf_rep2 (WA52_2-2)
Sample type RNA
 
Source name Wild type whole embryo, 52hpf, pool of ten embryos
Organism Danio rerio
Characteristics Strain: AB
Genotype: Wild-type
Age: 52hpf
Tissue: Whole embryo
Biomaterial provider Yuk Fai Leung/John E. Dowling
Treatment protocol Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate.
Growth protocol Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
Label Biotin
Label protocol Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Wild type whole embryos, 52hpf, pool of ten embryos, biological replicate 2
Data processing The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
 
Submission date Aug 24, 2007
Last update date Aug 14, 2011
Contact name Yuk Fai Leung
E-mail(s) yfleung@purdue.edu
Phone 765-496-3153
Organization name Purdue University
Department Biological Sciences
Lab Leung
Street address 915 W. State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL1319
Series (1)
GSE8874 Factorial Microarray Analysis of Zebrafish Retinal Development

Data table header descriptions
ID_REF
VALUE The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.

Data table
ID_REF VALUE
AFFX-BioB-3_at 188.116
AFFX-BioB-5_at 290.166
AFFX-BioB-M_at 227.46
AFFX-BioC-3_at 668.485
AFFX-BioC-5_at 642.558
AFFX-BioDn-3_at 2338.45
AFFX-BioDn-5_at 1218.68
AFFX-CreX-3_at 7754.5
AFFX-CreX-5_at 6229.73
AFFX-DapX-3_at 344.942
AFFX-DapX-5_at 58.3203
AFFX-DapX-M_at 120.107
AFFX-Dr-AB076373-1_at 31.3652
AFFX-Dr-acta1-3_at 16419.8
AFFX-Dr-acta1-5_at 80.3393
AFFX-Dr-acta1-5_x_at 3605.37
AFFX-Dr-acta1-M_at 11593
AFFX-Dr-AF292559-1_at 25.6222
AFFX-Dr-AF292559-2_s_at 30.1287
AFFX-Dr-AF292559-3_s_at 21.0019

Total number of rows: 15617

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM224795.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM224795.EXP.gz 514 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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