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Sample GSM224796 Query DataSets for GSM224796
Status Public on Aug 04, 2008
Title WA_52hpf_rep3 (WA52_2-3-2)
Sample type RNA
 
Source name Wild type whole embryo, 52hpf, pool of ten embryos
Organism Danio rerio
Characteristics Strain: AB
Genotype: Wild-type
Age: 52hpf
Tissue: Whole embryo
Biomaterial provider Yuk Fai Leung/John E. Dowling
Treatment protocol Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate.
Growth protocol Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
Label Biotin
Label protocol Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Wild type whole embryos, 52hpf, pool of ten embryos, biological replicate 3
Data processing The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
 
Submission date Aug 24, 2007
Last update date Aug 14, 2011
Contact name Yuk Fai Leung
E-mail(s) yfleung@purdue.edu
Phone 765-496-3153
Organization name Purdue University
Department Biological Sciences
Lab Leung
Street address 915 W. State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL1319
Series (1)
GSE8874 Factorial Microarray Analysis of Zebrafish Retinal Development

Data table header descriptions
ID_REF
VALUE The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.

Data table
ID_REF VALUE
AFFX-BioB-3_at 159.409
AFFX-BioB-5_at 209.023
AFFX-BioB-M_at 175.238
AFFX-BioC-3_at 635.862
AFFX-BioC-5_at 610.189
AFFX-BioDn-3_at 2207.47
AFFX-BioDn-5_at 1206.3
AFFX-CreX-3_at 8182.64
AFFX-CreX-5_at 6235.87
AFFX-DapX-3_at 338.353
AFFX-DapX-5_at 60.0637
AFFX-DapX-M_at 159.12
AFFX-Dr-AB076373-1_at 37.8109
AFFX-Dr-acta1-3_at 17681.7
AFFX-Dr-acta1-5_at 72.4642
AFFX-Dr-acta1-5_x_at 5783.77
AFFX-Dr-acta1-M_at 11987.7
AFFX-Dr-AF292559-1_at 55.5084
AFFX-Dr-AF292559-2_s_at 32.8275
AFFX-Dr-AF292559-3_s_at 37.6479

Total number of rows: 15617

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM224796.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM224796.EXP.gz 514 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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