|
Status |
Public on Aug 04, 2008 |
Title |
WR_36hpf_rep2 (WR36_3-2) |
Sample type |
RNA |
|
|
Source name |
Wild type retina, 36hpf, pool of ten tissues
|
Organism |
Danio rerio |
Characteristics |
Strain: AB Genotype: Wild-type Age: 36hpf Tissue: Retina
|
Biomaterial provider |
Yuk Fai Leung/John E. Dowling
|
Treatment protocol |
Ten tissues were dissected from developing embryos according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate.
|
Growth protocol |
Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
|
Label |
Biotin
|
Label protocol |
Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
|
|
Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
Description |
Wild type retina, 36hpf, pool of ten tissues, biological replicate 2
|
Data processing |
The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
|
|
|
Submission date |
Aug 24, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Yuk Fai Leung |
E-mail(s) |
yfleung@purdue.edu
|
Phone |
765-496-3153
|
Organization name |
Purdue University
|
Department |
Biological Sciences
|
Lab |
Leung
|
Street address |
915 W. State Street
|
City |
West Lafayette |
State/province |
IN |
ZIP/Postal code |
47907 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE8874 |
Factorial Microarray Analysis of Zebrafish Retinal Development |
|