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Sample GSM224809 Query DataSets for GSM224809
Status Public on Aug 04, 2008
Title YA_36hpf_rep1 (YA36_5-1)
Sample type RNA
 
Source name Young mutant whole embryo, 36hpf, pool of ten embryos
Organism Danio rerio
Characteristics Strain: AB
Genotype: young
Age: 36hpf
Tissue: Whole embryo
Biomaterial provider Yuk Fai Leung/John E. Dowling
Treatment protocol Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate. The young mutants were staged by staging their wild-type siblings.
Growth protocol Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
Label Biotin
Label protocol Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Young mutant whole embryos, 36hpf, pool of ten embryos, biological replicate 1
Data processing The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
 
Submission date Aug 24, 2007
Last update date Aug 14, 2011
Contact name Yuk Fai Leung
E-mail(s) yfleung@purdue.edu
Phone 765-496-3153
Organization name Purdue University
Department Biological Sciences
Lab Leung
Street address 915 W. State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL1319
Series (1)
GSE8874 Factorial Microarray Analysis of Zebrafish Retinal Development

Data table header descriptions
ID_REF
VALUE The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.

Data table
ID_REF VALUE
AFFX-BioB-3_at 309.598
AFFX-BioB-5_at 463.485
AFFX-BioB-M_at 464.226
AFFX-BioC-3_at 1199.63
AFFX-BioC-5_at 1037.56
AFFX-BioDn-3_at 3499.28
AFFX-BioDn-5_at 2130.82
AFFX-CreX-3_at 9529.98
AFFX-CreX-5_at 8369.93
AFFX-DapX-3_at 247.06
AFFX-DapX-5_at 70.3965
AFFX-DapX-M_at 160.403
AFFX-Dr-AB076373-1_at 30.289
AFFX-Dr-acta1-3_at 16471.9
AFFX-Dr-acta1-5_at 91.8886
AFFX-Dr-acta1-5_x_at 4497.56
AFFX-Dr-acta1-M_at 11972.8
AFFX-Dr-AF292559-1_at 38.1481
AFFX-Dr-AF292559-2_s_at 12.965
AFFX-Dr-AF292559-3_s_at 1.08532

Total number of rows: 15617

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM224809.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM224809.EXP.gz 521 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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