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Sample GSM224810 Query DataSets for GSM224810
Status Public on Aug 04, 2008
Title YA_36hpf_rep2 (YA36_5-2)
Sample type RNA
 
Source name Young mutant whole embryo, 36hpf, pool of ten embryos
Organism Danio rerio
Characteristics Strain: AB
Genotype: young
Age: 36hpf
Tissue: Whole embryo
Biomaterial provider Yuk Fai Leung/John E. Dowling
Treatment protocol Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate. The young mutants were staged by staging their wild-type siblings.
Growth protocol Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
Label Biotin
Label protocol Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Young mutant whole embryos, 36hpf, pool of ten embryos, biological replicate 2
Data processing The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
 
Submission date Aug 24, 2007
Last update date Aug 14, 2011
Contact name Yuk Fai Leung
E-mail(s) yfleung@purdue.edu
Phone 765-496-3153
Organization name Purdue University
Department Biological Sciences
Lab Leung
Street address 915 W. State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL1319
Series (1)
GSE8874 Factorial Microarray Analysis of Zebrafish Retinal Development

Data table header descriptions
ID_REF
VALUE The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.

Data table
ID_REF VALUE
AFFX-BioB-3_at 185.519
AFFX-BioB-5_at 235.449
AFFX-BioB-M_at 197.37
AFFX-BioC-3_at 708.703
AFFX-BioC-5_at 658.793
AFFX-BioDn-3_at 2332.43
AFFX-BioDn-5_at 1091.06
AFFX-CreX-3_at 8945.8
AFFX-CreX-5_at 6249.32
AFFX-DapX-3_at 425.786
AFFX-DapX-5_at 48.0665
AFFX-DapX-M_at 141.344
AFFX-Dr-AB076373-1_at 39.9707
AFFX-Dr-acta1-3_at 17638.7
AFFX-Dr-acta1-5_at 77.5253
AFFX-Dr-acta1-5_x_at 4893.86
AFFX-Dr-acta1-M_at 12366.5
AFFX-Dr-AF292559-1_at 41.852
AFFX-Dr-AF292559-2_s_at 20.6655
AFFX-Dr-AF292559-3_s_at 0

Total number of rows: 15617

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM224810.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM224810.EXP.gz 516 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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