|
| Status |
Public on Aug 04, 2008 |
| Title |
YA_36hpf_rep2 (YA36_5-2) |
| Sample type |
RNA |
| |
|
| Source name |
Young mutant whole embryo, 36hpf, pool of ten embryos
|
| Organism |
Danio rerio |
| Characteristics |
Strain: AB
Genotype: young
Age: 36hpf
Tissue: Whole embryo
|
| Biomaterial provider |
Yuk Fai Leung/John E. Dowling
|
| Treatment protocol |
Ten embryos were collected according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283., and pooled as one biological replicate. The young mutants were staged by staging their wild-type siblings.
|
| Growth protocol |
Fish and embryos were maintained according to Westerfield, M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th edition. University of Oregon Press, Eugene, 2000.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the pooled samples according to: Leung YF, Dowling JE. Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish 2005:2:269-283.
|
| Label |
Biotin
|
| Label protocol |
Thirty-three nanograms of total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
| Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
| Description |
Young mutant whole embryos, 36hpf, pool of ten embryos, biological replicate 2
|
| Data processing |
The probe-level data were background adjusted, normalized and summarized by a dChip (http://www.dchip.org) algorithm using default parameters.
|
| |
|
| Submission date |
Aug 24, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Yuk Fai Leung |
| E-mail(s) |
yfleung@purdue.edu
|
| Phone |
765-496-3153
|
| Organization name |
Purdue University
|
| Department |
Biological Sciences
|
| Lab |
Leung
|
| Street address |
915 W. State Street
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE8874 |
Factorial Microarray Analysis of Zebrafish Retinal Development |
|
