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Sample GSM225486 Query DataSets for GSM225486
Status Public on Jun 20, 2008
Title Placenta tissue from patient with normal labor, replicate 15 of 26
Sample type RNA
 
Source name total RNA from placenta tissue
Organism Homo sapiens
Characteristics Placenta tissue
Growth protocol Patient material
Extracted molecule total RNA
Extraction protocol MagNa Pure Compact RNA Isolation Kit (Roche Applied Science)
Label Chemiluminescent Fluorescence (Digoxigenin)
Label protocol 0.5 micrograms of total RNA was processed into digoxigenen (DIG)- labeled cRNA using the Applied Biosystems NanoAmp™ RT-IVT Labeling kit (Applied Biosystems)
 
Hybridization protocol The labelled DIG-cRNA (10 micrograms per microarray) was then injected into each microarray hybridization chamber. Following hybridization at 55°C for 16 hours, the unbound material was washed from the microarrays. Features that retained bound DIG- labeled cRNA were visualized using the Applied Biosystems Chemiluminescent Detection Kit. Anti-GIG alkaline phosphatase (Roche Applied Science) was used to hydrolyze a chemiluminescence substrate to generate light at 458 nm.
Scan protocol Images were quantified using the integrated ABI1700 software
Description Biological replicate 16 of 43. Placenta tissue from patients with normal labour
Data processing The data were pre-processed using the R software (version 2.4.1). A quantile normalization (ABarray package) was carried out prior to further analysis. This comes in addition to the internal preprocessing and filtering carried out by the ABI 1700 software (http=//www.amebioscience.com/ABI1700.htm).
 
Submission date Aug 29, 2007
Last update date Nov 13, 2015
Contact name Ruth Hracky Paulssen
E-mail(s) ruthp@fagmed.uit.no
Phone +47 77645480
Fax +47 77644650
URL http://www.unn.no/labforum
Organization name Universitetet i Tromsø
Department Institute of Clinical Medicine
Lab Molecular Medical Research
Street address MH-Bygget Breivika
City Tromsø
ZIP/Postal code N-9037
Country Norway
 
Platform ID GPL2986
Series (1)
GSE10588 Placental gene expression in severe preeclampsia.
Relations
Reanalyzed by GSE75010

Data table header descriptions
ID_REF Probe ID (from manufacturer)
VALUE Quantile normalized value of raw signal count. The raw signal value is the corrected, background subtracted measurement of chemiluminescent signal, as outputted by the ABI 1700 software.
RAW_VALUE Raw signal count
Flags Value other than zero indicates a degree of unrealibility of data (See ABI1700 Manual)
S_N Measurement uncertainty of the probe signal.
SDEV Standard deviation of signal count data

Data table
ID_REF VALUE RAW_VALUE Flags S_N SDEV
156427 355.060274662541 367.97 1 0.31 367.97
139282 239.560808357559 237.9 0 1.68 141.74
131316 1184.22304121222 1322.76 0 4.58 289.01
235751 3183.24906158605 3486.34 0 19.01 183.44
696770 776.564898896269 860.99 0 8.46 101.77
212224 71068.2366616332 69779.56 0 96.03 726.67
108888 2913.33623127554 3200.58 0 14.89 214.92
708044 823.337305500887 919.86 0 2.8 328.07
178946 192.700620362998 186.89 0 1.93 96.68
233299 19935.9654571555 20123.77 0 75.83 265.39
179411 592.003877688379 647.58 0 4.65 139.38
220912 357.89105511633 371.03 1 0.81 371.03
115338 36728.8216093521 36613.46 0 80.49 454.91
698913 545.935793699047 595.89 0 5.31 112.17
206927 2384.56116781427 2649.85 0 18.01 147.17
139705 263.319238366359 263.38 1 -0.65 263.38
110723 326.609331892845 335.19 0 3.54 94.73
223159 360.151463974195 373.21 0 3.48 107.38
705915 10956.0298270905 11201.82 0 46.52 240.8
135547 3160.40106104393 3462.08 0 21.83 158.58

Total number of rows: 32878

Table truncated, full table size 1474 Kbytes.




Supplementary file Size Download File type/resource
GSM225486.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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