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Sample GSM225488 Query DataSets for GSM225488
Status Public on Jun 20, 2008
Title Placenta tissue from patient with normal labor, replicate 17 of 26
Sample type RNA
 
Source name total RNA from placenta tissue
Organism Homo sapiens
Characteristics Placenta tissue
Growth protocol Patient material
Extracted molecule total RNA
Extraction protocol MagNa Pure Compact RNA Isolation Kit (Roche Applied Science)
Label Chemiluminescent Fluorescence (Digoxigenin)
Label protocol 0.5 micrograms of total RNA was processed into digoxigenen (DIG)- labeled cRNA using the Applied Biosystems NanoAmp™ RT-IVT Labeling kit (Applied Biosystems)
 
Hybridization protocol The labelled DIG-cRNA (10 micrograms per microarray) was then injected into each microarray hybridization chamber. Following hybridization at 55°C for 16 hours, the unbound material was washed from the microarrays. Features that retained bound DIG- labeled cRNA were visualized using the Applied Biosystems Chemiluminescent Detection Kit. Anti-GIG alkaline phosphatase (Roche Applied Science) was used to hydrolyze a chemiluminescence substrate to generate light at 458 nm.
Scan protocol Images were quantified using the integrated ABI1700 software
Description Biological replicate 18 of 43. Placenta tissue from patients with normal labour
Data processing The data were pre-processed using the R software (version 2.4.1). A quantile normalization (ABarray package) was carried out prior to further analysis. This comes in addition to the internal preprocessing and filtering carried out by the ABI 1700 software (http=//www.amebioscience.com/ABI1700.htm).
 
Submission date Aug 29, 2007
Last update date Nov 13, 2015
Contact name Ruth Hracky Paulssen
E-mail(s) ruthp@fagmed.uit.no
Phone +47 77645480
Fax +47 77644650
URL http://www.unn.no/labforum
Organization name Universitetet i Tromsø
Department Institute of Clinical Medicine
Lab Molecular Medical Research
Street address MH-Bygget Breivika
City Tromsø
ZIP/Postal code N-9037
Country Norway
 
Platform ID GPL2986
Series (1)
GSE10588 Placental gene expression in severe preeclampsia.
Relations
Reanalyzed by GSE75010

Data table header descriptions
ID_REF Probe ID (from manufacturer)
VALUE Quantile normalized value of raw signal count. The raw signal value is the corrected, background subtracted measurement of chemiluminescent signal, as outputted by the ABI 1700 software.
RAW_VALUE Raw signal count
Flags Value other than zero indicates a degree of unrealibility of data (See ABI1700 Manual)
S_N Measurement uncertainty of the probe signal.
SDEV Standard deviation of signal count data

Data table
ID_REF VALUE RAW_VALUE Flags S_N SDEV
156427 573.859338214242 638.88 0 1.22 525.56
139282 263.497314097762 337.56 1 -0.2 337.56
131316 2846.63494736551 2663.65 0 6.51 409.03
235751 2422.43883590464 2292.5 0 8.55 268.02
696770 1051.55761176578 1069.88 0 9.59 111.57
212224 68763.4103750113 58404.15 0 88.71 658.34
108888 1916.27005717605 1832.53 0 6.27 292.11
708044 420.471939064077 497.96 1 0.12 497.96
178946 413.741666392966 491.49 0 4.42 111.24
233299 27705.3407642323 23006.61 0 54.62 421.22
179411 693.022576943617 748.61 0 5.91 126.64
220912 228.223046859760 296.32 1 -0.06 296.32
115338 33712.0836495727 28218.65 0 54.1 521.62
698913 870.041949587305 903.96 0 2.67 339.17
206927 7625.71759101305 6723.11 0 13.05 515.27
139705 194.397587611469 251.28 1 -0.56 251.28
110723 91.3159539002164 125.62 0 1.16 108.13
223159 7026.15683512822 6216.84 32832 20.51 303.18
705915 9676.92956349373 8466.02 0 42.16 200.8
135547 6836.9838136214 6051.42 0 11.22 539.11

Total number of rows: 32878

Table truncated, full table size 1474 Kbytes.




Supplementary file Size Download File type/resource
GSM225488.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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