|
Status |
Public on Nov 04, 2007 |
Title |
WGACON-124 |
Sample type |
RNA |
|
|
Source name |
neuropathologically normal frozen temporal cortex
|
Organism |
Homo sapiens |
Characteristics |
GENDER_male,AGE_68,PMI_16,TRANSDET_WHOLE_0.441,TRANSDET_CORTEX_0.754,HYBDATE_8.31.2006,BRAINBANK_G
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA (Qiagen Rneasy Lipid Tissue cat #cat 74804, Qiagen Rnase free dnase set- cat # 79254), genomic DNA (Qiagen Dneasy kit cat # 69504)
|
Label |
biotin
|
Label protocol |
RNA: per ambion manual for cat #IL1791, biotinylated cRNA created from in vitro translation targeting poly A tail, cy3 used to image RNA signal DNA: biotin labeled as per Affymetrix protocol for cat # 900753/900754
|
|
|
Hybridization protocol |
RNA: Illumina Whole Genome Gene Expression Rev. F (Illumina Doc #11763837);DNA: Allele Specific Hybridization, Affymetrix Mapping 500K Assay Manual (Affymetrix P/N 701930 Rev. 3)
|
Scan protocol |
RNA: Illumina Beadstation; DNA: Genechip Scanner 3000
|
Description |
Human Cortex from neurologically normal control, Caucasian, male.
|
Data processing |
RNA: Beadstudio software v 1.5.0.34, rank invariant normalization to extract, log10 transformation before analysis
|
|
|
Submission date |
Aug 29, 2007 |
Last update date |
Apr 01, 2009 |
Contact name |
Amanda Myers |
Organization name |
Miller School of Medicine, University of Miami
|
Department |
Psychiatry and Behavioral Sciences
|
Street address |
1600 NW 10th Ave
|
City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
|
|
Platform ID |
GPL2700 |
Series (2) |
GSE8919 |
A Survey of Genetic Human Cortical Gene Expression |
GSE15222 |
Genetic control of human brain transcript expression in Alzheimer’s disease. |
|