|
| Status |
Public on Aug 31, 2017 |
| Title |
patient 4 16GSQ-0 |
| Sample type |
RNA |
| |
|
| Source name |
effective case_before treatment
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: type 2 diabetes with obesity and hyperlipidemia
sample group: effective case, before treatment
subject id: patient4
gender: female
age (yrs): 57
tissue: serum
|
| Treatment protocol |
patients undergoing the treatment of “Jiangtang tiaozhi” formula for 6 months at the Guang’anmen Hospital (Beijing, China) between February1 and July 30, 2012.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from homogenized samples with TRIzol reagent according to the manufacturer’s instruction.
|
| Label |
cy3
|
| Label protocol |
Sample labeling was performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.
|
| |
|
| Hybridization protocol |
The GeneFilters were prehybridized for 2 h at 51C with 0.5 mg/ml of poly-dA (Invitrogen) and 0.5 mg/ml Cot-1 DNA (Invitrogen) in 10 ml of AlkPhos DIRECT hybridization buffer (Amersham Bioscience) and then hybridized for 17 h at 51C with the denatured radi
|
| Scan protocol |
The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
|
| Description |
SAMPLE 4
|
| Data processing |
The raw data were re-scaled to account for the differences in individual hybridization intensities. We employed a rank-invariant scaling method to select genes (Tseng et al., 2001), which were then used for fitting of a non-linear normalization curve. A
|
| |
|
| Submission date |
Aug 04, 2016 |
| Last update date |
Aug 31, 2017 |
| Contact name |
Lisha He |
| E-mail(s) |
395404560@qq.com
|
| Phone |
+86-18810378782
|
| Organization name |
Guang’anmen Hospital, China Academy of Chinese Medical Sciences
|
| Department |
Endocrine Department
|
| Street address |
No 5. Beixiange Street, Xicheng District
|
| City |
Beijing |
| ZIP/Postal code |
100053 |
| Country |
China |
| |
|
| Platform ID |
GPL16956 |
| Series (1) |
| GSE85192 |
lncRNA Expression Signatures in Response to Jiangtang Tiaozhi Formular in T2DM with Obesity and Hyperlipidemia |
|
