|
| Status |
Public on Nov 04, 2007 |
| Title |
WGACON-226 |
| Sample type |
RNA |
| |
|
| Source name |
neuropathologically normal frozen frontal temporal cortex
|
| Organism |
Homo sapiens |
| Characteristics |
GENDER_male,AGE_86,PMI_7,TRANSDET_WHOLE_0.445,TRANSDET_CORTEX_0.769,HYBDATE_3.9.2006,BRAINBANK_N
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA (Qiagen Rneasy Lipid Tissue cat #cat 74804, Qiagen Rnase free dnase set- cat # 79254)
|
| Label |
biotin
|
| Label protocol |
RNA: per ambion manual for cat #IL1791, biotinylated cRNA created from in vitro translation targeting poly A tail, cy3 used to image RNA signal
|
| |
|
| Hybridization protocol |
RNA: Illumina Whole Genome Gene Expression Rev. F (Illumina Doc #11763837)
|
| Scan protocol |
RNA: Illumina Beadstation
|
| Description |
Human Cortex from neurologically normal control, Caucasian, male.
|
| Data processing |
RNA: Beadstudio software v 1.5.0.34, rank invariant normalization to extract, log10 transformation before analysis
|
| |
|
| Submission date |
Aug 30, 2007 |
| Last update date |
Apr 01, 2009 |
| Contact name |
Amanda Myers |
| Organization name |
Miller School of Medicine, University of Miami
|
| Department |
Psychiatry and Behavioral Sciences
|
| Street address |
1600 NW 10th Ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL2700 |
| Series (2) |
| GSE8919 |
A Survey of Genetic Human Cortical Gene Expression |
| GSE15222 |
Genetic control of human brain transcript expression in Alzheimer’s disease. |
|
