|
Status |
Public on Mar 02, 2011 |
Title |
Human kidney_1 week posttransplantation |
Sample type |
RNA |
|
|
Source name |
Human liver, 1 week posttransplantation
|
Organism |
Homo sapiens |
Characteristics |
Female, 64 years, in combined liver-kidney transplantation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Mini kit for total RNA purification according to manufacturers instructions.
|
Label |
DIG-dUTP
|
Label protocol |
500ng of total RNA from each sample was reversely transcribed, amplified and DIG-labelled (DIG-dUTP; Roche, Germany), using the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit.
|
|
|
Hybridization protocol |
Fifteen μg of DIG-labelled cRNA was hybridised to the Applied Biosystems Human Genome Survey Microarray v.2.0 according to the manufacturer’s instructions.
|
Scan protocol |
The chemiluminescent signal detection, image acquisition and image analysis of the microarrays were performed on the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer.
|
Description |
Human kidney 1 week posttransplantation in combined liver-kidney transplantation.
|
Data processing |
Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software.
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|
|
Submission date |
Sep 04, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Madeleine Ingelsten |
Organization name |
Gothenburg university
|
Department |
Renal Center
|
Lab |
Wallenberg lab, lab 8
|
Street address |
Bruna Straket 16
|
City |
Gothenburg |
ZIP/Postal code |
413 45 |
Country |
Sweden |
|
|
Platform ID |
GPL2986 |
Series (1) |
GSE8941 |
Human auxiliary liver and combined kidney transplantation |
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