|
| Status |
Public on Mar 02, 2011 |
| Title |
Human native liver_1 week posttransplantation of auxiliary liver |
| Sample type |
RNA |
| |
|
| Source name |
Human native liver, 1 week posttransplantation of auxiliary liver
|
| Organism |
Homo sapiens |
| Characteristics |
Female, 64 years, in combined liver-kidney transplantation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini kit for total RNA purification according to manufacturers instructions.
|
| Label |
DIG-dUTP
|
| Label protocol |
500ng of total RNA from each sample was reversely transcribed, amplified and DIG-labelled (DIG-dUTP; Roche, Germany), using the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit.
|
| |
|
| Hybridization protocol |
Fifteen μg of DIG-labelled cRNA was hybridised to the Applied Biosystems Human
Genome Survey Microarray v.2.0 according to the manufacturer’s instructions.
|
| Scan protocol |
The chemiluminescent signal detection, image acquisition and image analysis
of the microarrays were performed on the Applied Biosystems 1700 Chemiluminescent
Microarray Analyzer.
|
| Description |
Human native liver 1 week posttransplantation of auxiliary liver.
|
| Data processing |
Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software.
|
| |
|
| Submission date |
Sep 04, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Madeleine Ingelsten |
| Organization name |
Gothenburg university
|
| Department |
Renal Center
|
| Lab |
Wallenberg lab, lab 8
|
| Street address |
Bruna Straket 16
|
| City |
Gothenburg |
| ZIP/Postal code |
413 45 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE8941 |
Human auxiliary liver and combined kidney transplantation |
|
