|
| Status |
Public on Aug 15, 2008 |
| Title |
WT colon repl2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type mouse colon
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini kit (QIAGEN, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA amplified with TargetAmp 1-round Aminoallyl-aRNA Amplication Kit 101 (Epicentre, WI) (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the mouse MOE-430 GeneChip array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Description |
gene expression data from adult mouse colon
|
| Data processing |
The data was analyzed with GeneSpring version 7.3 using RMA normalization with a custom CDF file(Mm430_Mm_REFSEQ_8), then normalized to the REF colon repl1 and the mean of the 3 WT colon samples
|
| |
|
| Submission date |
Sep 04, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Xiaonan Han |
| E-mail(s) |
xiaonan.han@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Gastroenterology
|
| Lab |
MLC2010
|
| Street address |
3333 Burnet Ave.
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL5759 |
| Series (1) |
| GSE8942 |
Mouse colon expression in wild type and STAT5b deficient mice |
|
