|
| Status |
Public on Apr 03, 2008 |
| Title |
Hippocampi from kainate treated mutant animal, biological rep. 3 |
| Sample type |
RNA |
| |
|
| Source name |
Pooled hippocampi from 3 kainate treated mutant animals
|
| Organism |
Mus musculus |
| Characteristics |
Mixed background C57Bl6 and 129, Cre+, Creb1 flox/flox, Crem -/-
|
| Treatment protocol |
Intra peritoneal injection of 20 mg/kg kainate, animals were sacrificed one hour after the injection
|
| Growth protocol |
5 to 6 week-old littermate male and female mice were single-caged and handled for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hippocampi were removed and placed in RNAlater (Ambion) overnight. Total RNA was prepared with Absolutely RNA Miniprep kit (Stratagene) and its quality was assessed on RNA LabChips (Agilent). RNA samples from 3 animals were pooled before labelling.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on mouse MG-U74Cv2 arrays. Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mutant animals treated with kainate
|
| Data processing |
Analysis of array data was performed using the R 2.4.1/Bioconductor 1.9 (Gentleman et al. 2004) . Data was normalized and expression values were computed using the gcrma method.
|
| |
|
| Submission date |
Sep 04, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Jan Rodriguez Parkitna |
| E-mail(s) |
j.rodriguez@dkfz.de
|
| Phone |
+49 6221 423437
|
| Organization name |
German Cancer Research Center
|
| Lab |
Molecular Biology of the Cell I
|
| Street address |
Im Neuenheimer Feld 581
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL83 |
| Series (2) |
| GSE8947 |
Effect of loss of CREB and CREM on kainate-induced gene expression in the hippocampus (MG-U74C). |
| GSE8948 |
Effect of loss of CREB and CREM on cocaine-induced gene expression in the striatum |
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