|
| Status |
Public on Aug 17, 2009 |
| Title |
Mock-infected SCID mouse liver_Sample142 |
| Sample type |
RNA |
| |
|
| Source name |
Mock-infected SCID mouse liver; control.
|
| Organism |
Mus musculus |
| Characteristics |
SCID mouse, male, 7 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from E. chaffeensis-infected or uninfected mouse liver using RNeasy Mini Kit (QIAGEN).
|
| Label |
phycoerythrin
|
| Label protocol |
About 8 ug of each RNA sample was applied to double-strand cDNA preparation using GeneChip One-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA). Biotin-labeled cRNA was generated from the cDNA using GeneChip IVT Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled cRNA was hybridized to GeneChip Mouse Genome 430 2.0 Array (Affymetrix) containing over 39,000 element-spotted murine cDNA microarrays.
|
| Scan protocol |
After washing and staining with streptoavidin phycoerythrin, the array was scanned with GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
3 mice were used for this experiment.
|
| Data processing |
Data analysis was performed with Partek Discovery Suite (Partek Inc., St. Louis, MO). The average differences obtained with probes derived from liver samples infected with each of three strains of E. chaffeensis, were base-lined against average differences obtained from sham-infected liver samples to generate the -fold difference value for each gene call.
|
| |
|
| Submission date |
Sep 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Koshiro Miura |
| E-mail(s) |
miura.8@osu.edu
|
| Phone |
614 247 7683
|
| Organization name |
The Ohio State University
|
| Department |
Veterinary Biosciences
|
| Street address |
1900 Coffey Rd
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8966 |
Liver Transcriptome Profiles Associated with Strain-Specific Ehrlichia chaffeensis–induced Hepatitis |
|
