|
Status |
Public on Dec 01, 2007 |
Title |
C57Bl/6 AM 2hr_post e7 conidia, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Alveolar Macrophage (AM) and control untreated
|
Organism |
Mus musculus |
Characteristics |
AM collected from C57Bl/6, 2 hours after exposure to Aspergillus fumigatus conidia
|
Treatment protocol |
Sample AM collected 2 hours after in vivo incubation with conidia
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy RNA extraction kit (Quiagen) was performed on AM harvested fresh according to the manufacturer's instructions from mouse lungs.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard two-cycle Affymetrix protocol from 100 nanograms total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr_at 45C on GeneChip Mouse GeneChip 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GCOS Scanner 3000.
|
Description |
Gene expression timecourse from AM following in vivo exposure to A. fumigatus conidia.
|
Data processing |
Data was analyzed with Agilent Genespring GX v. 7.3.1 using Affymetrix GCOS generated flag data from scaled CHP files, and RMA-normalized CEL files.
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Submission date |
Sep 10, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Kate McInnerney |
E-mail(s) |
kmcinnerney@montana.edu
|
Phone |
406-994-5666
|
Fax |
406-994-4926
|
URL |
http://cores.montana.edu
|
Organization name |
Montana State University
|
Department |
Microbiology
|
Lab |
Functional Genomics Facility
|
Street address |
109 Lewis Hall
|
City |
Bozeman |
State/province |
MT |
ZIP/Postal code |
59717 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE8997 |
XX hour expression data of AM from C57Bl/6 or gp91phox-/- mice following exposure to conidia or polystyrene beads |
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