|
| Status |
Public on Dec 01, 2007 |
| Title |
C57Bl/6 AM 4hrs after mock txmt, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar Macrophage (AM)
|
| Organism |
Mus musculus |
| Characteristics |
AM collected from C57Bl/6 and gp91phox-/-, 4 hour after mock inoculation (no conidia)
|
| Treatment protocol |
Sample AM collected 4 hours after in vivo incubation with conidia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy RNA extraction kit (Quiagen) was performed on AM harvested fresh according to the manufacturer's instructions from mouse lungs.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard two-cycle Affymetrix protocol from 100 nanograms total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr_at 45C on GeneChip Mouse GeneChip 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCOS Scanner 3000.
|
| Description |
Gene expression timecourse from AM following in vivo exposure to A. fumigatus conidia.
|
| Data processing |
Data was analyzed with Agilent Genespring GX v. 7.3.1 using Affymetrix GCOS generated flag data from scaled CHP files, and RMA-normalized CEL files.
|
| |
|
| Submission date |
Sep 10, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
|
| Phone |
406-994-5666
|
| Fax |
406-994-4926
|
| URL |
http://cores.montana.edu
|
| Organization name |
Montana State University
|
| Department |
Microbiology
|
| Lab |
Functional Genomics Facility
|
| Street address |
109 Lewis Hall
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE8997 |
XX hour expression data of AM from C57Bl/6 or gp91phox-/- mice following exposure to conidia or polystyrene beads |
|
