NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM228786 Query DataSets for GSM228786
Status Public on Nov 19, 2007
Title Normal liver_WT-rep1
Sample type RNA
 
Source name Normal liver of WT
Organism Mus musculus
Characteristics C57BL/6
Male
Age: 20 months
Growth protocol Mice were bred and housed in the Clinical Mouse Institute, ICS (Strasbourg, France) according to guidelines in compliance with the European legislation on care and use of laboratory anaimals.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNeasy Kit from QIAGEN.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix Scanner GCS3000
Description Gene expression data from livers of adult wild-type mice
Data processing The data were analyzed with Affymetrix GCOS software version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Sep 11, 2007
Last update date Aug 28, 2018
Contact name RĂ©gine Losson
E-mail(s) losson@igbmc.u-strasbg.fr
Organization name IGBMC
Street address 1 rue Laurent Fries
City ILLKIRCH CEDEX
ZIP/Postal code 67404
Country France
 
Platform ID GPL1261
Series (1)
GSE9012 Expression data from spontaneous liver tumors of Trim24/TIF1a-null mice.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 114.244 P 0.00227496
AFFX-BioB-M_at 153.882 P 0.000126798
AFFX-BioB-3_at 108.032 P 0.000126798
AFFX-BioC-5_at 236.434 P 7.00668e-05
AFFX-BioC-3_at 260.573 P 7.00668e-05
AFFX-BioDn-5_at 337.678 P 5.16732e-05
AFFX-BioDn-3_at 1185.04 P 5.16732e-05
AFFX-CreX-5_at 3921.54 P 6.02111e-05
AFFX-CreX-3_at 4983.47 P 4.42873e-05
AFFX-DapX-5_at 6.65095 A 0.287743
AFFX-DapX-M_at 11.6462 A 0.205732
AFFX-DapX-3_at 0.752021 A 0.969024
AFFX-LysX-5_at 5.66787 A 0.227636
AFFX-LysX-M_at 1.35462 A 0.916408
AFFX-LysX-3_at 1.68485 A 0.5
AFFX-PheX-5_at 1.21375 A 0.485109
AFFX-PheX-M_at 0.951364 A 0.953518
AFFX-PheX-3_at 3.90885 A 0.60308
AFFX-ThrX-5_at 2.35477 A 0.574044
AFFX-ThrX-M_at 6.87638 A 0.440646

Total number of rows: 45101

Table truncated, full table size 1370 Kbytes.




Supplementary file Size Download File type/resource
GSM228786.CEL.gz 6.0 Mb (ftp)(http) CEL
GSM228786.CHP.gz 11.0 Mb (ftp)(http) CHP
GSM228786.EXP.gz 504 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap