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Sample GSM228789 Query DataSets for GSM228789
Status Public on Nov 19, 2007
Title Normal liver_WT-rep4
Sample type RNA
 
Source name Normal liver of WT
Organism Mus musculus
Characteristics C57BL/6
Male
Age: 20 months
Growth protocol Mice were bred and housed in the Clinical Mouse Institute, ICS (Strasbourg, France) according to guidelines in compliance with the European legislation on care and use of laboratory anaimals.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNeasy Kit from QIAGEN.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix Scanner GCS3000
Description Gene expression data from livers of adult wild-type mice
Data processing The data were analyzed with Affymetrix GCOS software version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Sep 11, 2007
Last update date Aug 28, 2018
Contact name RĂ©gine Losson
E-mail(s) losson@igbmc.u-strasbg.fr
Organization name IGBMC
Street address 1 rue Laurent Fries
City ILLKIRCH CEDEX
ZIP/Postal code 67404
Country France
 
Platform ID GPL1261
Series (1)
GSE9012 Expression data from spontaneous liver tumors of Trim24/TIF1a-null mice.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 76.8554 P 0.00179591
AFFX-BioB-M_at 165.421 P 0.0020226
AFFX-BioB-3_at 83.024 P 0.00359458
AFFX-BioC-5_at 224.414 P 4.42873e-05
AFFX-BioC-3_at 216.564 P 6.02111e-05
AFFX-BioDn-5_at 217.844 P 0.000146581
AFFX-BioDn-3_at 821.691 P 4.42873e-05
AFFX-CreX-5_at 2583.42 P 4.42873e-05
AFFX-CreX-3_at 4139.68 P 4.42873e-05
AFFX-DapX-5_at 12.5363 P 0.0396608
AFFX-DapX-M_at 2.27149 A 0.60308
AFFX-DapX-3_at 2.16455 A 0.544587
AFFX-LysX-5_at 7.67397 A 0.102086
AFFX-LysX-M_at 2.5068 A 0.772364
AFFX-LysX-3_at 1.25465 A 0.574044
AFFX-PheX-5_at 4.0319 A 0.617401
AFFX-PheX-M_at 1.13015 A 0.883887
AFFX-PheX-3_at 6.24237 A 0.544587
AFFX-ThrX-5_at 6.14339 A 0.48511
AFFX-ThrX-M_at 1.11083 A 0.876428

Total number of rows: 45101

Table truncated, full table size 1370 Kbytes.




Supplementary file Size Download File type/resource
GSM228789.CEL.gz 5.9 Mb (ftp)(http) CEL
GSM228789.CHP.gz 10.8 Mb (ftp)(http) CHP
GSM228789.EXP.gz 356 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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