|
| Status |
Public on Jun 25, 2008 |
| Title |
WT_BoneMarrowMacrophages_LPS-stimulated_1hour_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse bone marrow macrophages, LPS stimulated, 1 hour
|
| Organism |
Mus musculus |
| Characteristics |
bone marrow macrophages from 1 female and 2 male wild type Balb/c mice
|
| Biomaterial provider |
Novartis Institutes for BioMedical Research, Models of Disease Center
|
| Treatment protocol |
stimulation with 1 ug/ml LPS for 1 hour
|
| Growth protocol |
cells were grown in macrophage-SFM medium (Gibco) containing 10% FCS and 30% L-929R-cell-conditioned medium at 37°C in a humidified CO2 (5%) incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy protocol following the manufacturer's recommendations
|
| Label |
biotin/SAPE
|
| Label protocol |
NuGEN Ovation protocol following the manufacturer's recommendations
|
| |
|
| Hybridization protocol |
see Affymetrix GeneChip Expression Analysis Manual and Microarray Analysis Suite 5.0 Software. Affymetrix. Reference 701001rev2. 2001
|
| Scan protocol |
see Affymetrix GeneChip Expression Analysis Manual and Microarray Analysis Suite 5.0 Software. Affymetrix. Reference 701001rev2. 2001
Affymetrix 11 µm feature
parameter settings: alpha 1 = 0.05, alpha 2 = 0.065, tau = 0.15
|
| Description |
no additional description
|
| Data processing |
Affymetrix MAS 5.0, trimmed mean (trimming off the top and bottom 2%) set to 150
|
| |
|
| Submission date |
Sep 13, 2007 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Anton Glück |
| E-mail(s) |
anton.glueck@novartis.com
|
| Organization name |
Novartis Institutes for BioMedical Research
|
| Street address |
WSJ-386.13.50
|
| City |
Basel |
| ZIP/Postal code |
4002 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9037 |
response to LPS of WT and IRAK4 kinase dead mouse bone marrow macrophages |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
