|
Status |
Public on Jun 25, 2008 |
Title |
WT_BoneMarrowMacrophages_LPS-stimulated_1hour_rep2 |
Sample type |
RNA |
|
|
Source name |
WT mouse bone marrow macrophages, LPS stimulated, 1 hour
|
Organism |
Mus musculus |
Characteristics |
bone marrow macrophages from 1 female and 2 male wild type Balb/c mice
|
Biomaterial provider |
Novartis Institutes for BioMedical Research, Models of Disease Center
|
Treatment protocol |
stimulation with 1 ug/ml LPS for 1 hour
|
Growth protocol |
cells were grown in macrophage-SFM medium (Gibco) containing 10% FCS and 30% L-929R-cell-conditioned medium at 37°C in a humidified CO2 (5%) incubator
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy protocol following the manufacturer's recommendations
|
Label |
biotin/SAPE
|
Label protocol |
NuGEN Ovation protocol following the manufacturer's recommendations
|
|
|
Hybridization protocol |
see Affymetrix GeneChip Expression Analysis Manual and Microarray Analysis Suite 5.0 Software. Affymetrix. Reference 701001rev2. 2001
|
Scan protocol |
see Affymetrix GeneChip Expression Analysis Manual and Microarray Analysis Suite 5.0 Software. Affymetrix. Reference 701001rev2. 2001 Affymetrix 11 µm feature parameter settings: alpha 1 = 0.05, alpha 2 = 0.065, tau = 0.15
|
Description |
no additional description
|
Data processing |
Affymetrix MAS 5.0, trimmed mean (trimming off the top and bottom 2%) set to 150
|
|
|
Submission date |
Sep 13, 2007 |
Last update date |
Aug 02, 2019 |
Contact name |
Anton Glück |
E-mail(s) |
anton.glueck@novartis.com
|
Organization name |
Novartis Institutes for BioMedical Research
|
Street address |
WSJ-386.13.50
|
City |
Basel |
ZIP/Postal code |
4002 |
Country |
Switzerland |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9037 |
response to LPS of WT and IRAK4 kinase dead mouse bone marrow macrophages |
|
Relations |
Reanalyzed by |
GSE119085 |
Reanalyzed by |
GSE135324 |